Alpha and Beta-diversity of Microbial Communities Associated to Plant Disease Suppressive Functions of On-farm Green Composts

: Green waste composts are obtained from agricultural production chains; their suppressive properties are increasingly being developed as a promising biological control option in the management of soil-borne phytopathogens. The wide variety of microbes harbored in the compost ecological niches may regulate suppressive functions through not yet fully known underlying mechanisms. This study investigates alpha- and beta-diversity of the compost microbial communities, as indicators of the biological features. Our green composts displayed a di ﬀ erential pattern of suppressiveness over the two assayed pathosystems. Fungal and bacterial densities, as well as catabolic and enzyme functionalities did not correlate with the compost control e ﬃ cacy on cress disease. Di ﬀ erences in the suppressive potential of composts can be better predicted by the variations in the community levels of physiological proﬁles indicating that functional alpha-diversity is more predictive than that which is calculated on terminal restriction fragments length polymorphisms (T-RFLPs) targeting the 16S rRNA gene. However, beta-diversity described by nMDS analysis of the Bray–Curtis dissimilarity allowed for separating compost samples into distinct functionally meaningful clusters and indicated that suppressiveness could be regulated by selected groups of microorganisms as major deterministic mechanisms. This study contributes to individuating new suitable characterization procedures applicable to the suppressive green compost chain.


Introduction
Soil-borne fungal diseases are among the main and most feared factors limiting agricultural production and economic sustainability: they are responsible for severe outbreaks that are difficult to eradicate without the massive use of high-impacting synthetic fungicides. Public concerns about environmental and health risks linked to the indiscriminate use of these are prompting the setup and implementation of effective nonchemical technologies for developing ecofriendly disease control methods that include biological, agronomic and genetic means. Compost may enhance the sustainability of intensive agricultural systems through its natural suppressiveness properties [1]. Suppressiveness occurs when compost interferes in the compatible plant-pathogen interaction, generating conditions unfavorable to the pathogenesis [2]. A quality compost may constitute the

Compost Collection
In this study, 13 on-farm composts (coded from C1 to C13), were used. The composts were obtained through composting of raw agricultural materials aerated first by mechanical turning, and on static piles by basal forced ventilation along 45-day active phase, followed by a two months-curing period. Feedstock used in compost piles were composed as follow: C1 -leafy vegetables, fennel and woodchips; C2 -maize, livestock waste and woodchips; C3 -leafy vegetables, basil, tomato, watermelon and woodchips; C4 -leafy vegetables, basil, watermelon and woodchips; C5 -leafy vegetables, basil, pumpkin and woodchips; C6 -leafy vegetables, basil and woodchips; C7 -leafy vegetables, basil, watermelon and woodchips; C8 -leafy vegetables, basil and woodchips; C9 -leafy vegetables, basil and woodchips; C10 -leafy vegetables, basil, pumpkin and woodchips; C11 -leafy vegetables, artichoke and woodchips; C12 -leafy vegetables, cabbage, walnut husk and woodchips; C13 -leafy vegetables, basil, sorghum, tomato, pumpkin and woodchips. During the thermophilic phase, pile heating reached up to 60 • C and stayed for about 4 days, to achieve biomasses sanitation. For all the microbiological analyses and for the DNA extraction, compost sampling was carried out by pooling and mixing10 subsamples taken by a small shovel at 0.5-1.0 m depth, from 10 different points of each compost pile (dimension 1.5 m × 30 m). Then, samples were stored at 4 • C, while for the molecular study, a representative aliquot was stored at −80 • C until DNA extraction.

Compost Suppressive Assay
Plant fungal pathogens used in this study were R. solani (AG-4) and S. minor [8], maintained on potato dextrose agar medium (PDA, Oxoid) in the fungi collection of CREA-Research centre for vegetables and ornamental crops (Pontecagnano Faiano, Italy). The ability of the different composts to suppress R. solani and S. minor damping-off was assessed by evaluating the L. sativum damping off occurrence in artificially inoculated amended media [2]. Potting-mixtures were prepared by amending Agriculture 2020, 10, 113 3 of 11 autoclaved soil peat with 20% (v/v) of both sterile (twice autoclaved) or not sterile compost, while pots fully filled only with sterilized peat were used as the nonamended control. The experimental design included five pots per treatment, and the experiment was repeated twice. After 7 days, incubation disease incidence was assessed as the percentage of damped-off seedlings and the compost control efficacy was calculated as described by Shin et al. [12] using the following formula: Control efficacy (%) = 100 − (100 × disease incidence of treatment/disease incidence of control).

Assessment of Compost Phytotoxicity
Compost phytotoxicity was assessed on cress seeds soaked with compost water extracts (at 50, 16.6 and 5 g L −1 ), by measuring germination and root elongation of seedlings and compared to the water control. The number of germinated seeds and the root length, recorded after 36 h, were used to calculate the germination index (GI%) with the following formula: GI% = [(n. germinated seeds on extracts/n. of germinated seeds on water) × (Mean root length on extracts/Mean root length on water)] × 100.

Population Counting
The abundance of culturable filamentous fungi and total bacteria in compost samples was evaluated by serial ten-fold (10 −1 to 10 −7 ) dilution method in three replicates with three independent samples. Fungi were counted on PDA (Oxoid) pH 6, and supplemented with 150 mg L −1 of nalidixic acid and 150 mg L −1 of streptomycin. Total bacteria were counted on selective medium (glucose 1 g L −1 , proteose peptone 3 g L −1 , yeast extract 1 g L −1 , K 2 PO 4 1 g L −1 , agar 15 g L −1 ) supplemented with actidione 100 mg L −1 . Peudomonads were counted on selective agar medium without iron, supplemented with actidione, while spore-forming Bacillus-like bacteria were counted by plating eluates previously heated at 90 • C for 10 min, on Nutrient Agar [8]. Population densities are reported as Log c.f.u. g −1 dry weight of compost.

Enzymatic and Metabolic Analysis
Fluorescein diacetate (FDA) hydrolysis was assessed on three independent 2.5 g compost samples, each supplemented with 15 mL of 0.2 M potassium phosphate buffer, pH 7.6. Enzymatic reaction was started by adding 0.5 mL FDA solution (2 mg mL −1 ). The sample was shaken for 2 h in an orbital incubator and hydrolysis reaction was stopped by adding 15 mL CHCl 3 /CH 3 OH (2:1 v/v). Following centrifugation (700 × g) and filtration of the aqueous phase, the absorbance of filtrates was measured at 490 nm by SpecrtoFlex 6600 (WTW, Weilheim, Germany). Blanks, without addition of FDA, were also included to correct for background absorbance and the amount of fluorescein release was determined against a calibration curve. Diversity in microbial metabolism was evaluated by BIOLOG EcoPlates™ (Biolog, Inc., Hayward, CA, USA) method, based on the discrimination of carbon substrate utilization ability. BIOLOG EcoPlates™ contain three replicate wells of 31 carbon substrates and a blank in triplicate. As the carbon source is utilized, the tetrazolium violet dye is reduced, developing a purple color. Compost water eluates prepared by vigorously shaking compost at ratio of 1:10 (w/v) for 15 min, were poured into each well of the microplates; then, the plates were incubated at 25 • C for 4 days in the dark and color development in each well was recorded, 96 h post inoculum, as the optical density (λ = 590 nm) of each well, using the Bio-Rad Microplate Reader 550 (Bio-rad, Inc., Hercules, CA, USA). The assay was conducted as previously described by Bartelt-Ryser et al. [13]. Average well color development (AWCD) was calculated as the sum of activities measured in all wells of each plate, divided by the 31 carbon sources.

T-RFLP Analysis
In order to analyze terminal restriction fragments length polymorphism (T-RFLP), DNA was extracted from composts using the DNeasy Power Soil kit (Qiagen, Germantown, MD, USA) following Agriculture 2020, 10, 113 4 of 11 the manufacturer's instructions. Samples with a high content of humic substances were processed by doing two steps with the inhibitor removal buffers designed to precipitate non-DNA organic and inorganic materials. Microbial 16S rDNA was PCR amplified with the oligonucleotide pair 27F (5'-AGAGTTTGATCCTGGCTCAG-3') and 1492R (5'-GGTTACCTTGTTACGACTT-3'), with the forward primer labeled with the fluorescent dye FAM (6-carboxyfluorescein) [14]. Reactions were carried out in a final volume of 100 µl with 10 ng of DNA template, 1 × PCR buffer containing 2 mM MgCl 2 , 0.2 mM nucleotide mix (abm), 1.25 units of Taq polymerase (DreamTaq, Thermo Scientific) and 2 µM of each primer. The amplicons were obtained with the following cycle: 1 × (94 for 3 min), 35 × (94 • C, 30 sec; 52 • C, 30sec; 72 • C, 90 sec), 1 × (72 • C, 10 min). Aliquots of 10 µl were visualized on 1.2% agarose gel stained with SYBR safe DNA gel stain (Thermo Fischer Scientific) to assess the presence and the quality of the amplicons before the digestion step. Three restriction enzymes with four-base recognition sequence were used in this work: AluI, HhaI, RsaI (New England Biolabs). A total of 100 ng of purified PCR product was separately digested with 10U of enzyme at 37 • C for 3 hours to produce 5' end-labeled 16S rDNA fragments. Complete digestions of PCR products have been verified by running an aliquot (10 µl) on an agarose gel, purified and then electrophoretically separated by an ABI Prism 3130 (Applied Biosystems). The lengths and fluorescens of the FAM-labeled terminal restriction fragments (t-RFs) were determined by comparison with an internal standard (ROX 500, Applied Biosystems) with a peak height detection of 40 units.

Biodiversity Assessment and Statistical Analysis
Catabolic and t-RFs-based microbial diversity were assessed by alpha-diversity descriptive indexes [15] on the data normalized across all values from the corresponding sample to account for run-to-run variations [16]. Richness (S) was determined by the number of peaks. Shannon diversity index (H ) was determined using the formula H = − (pi) (ln pi), while evenness (J ) was calculated as J = H /ln(S), and the Simpson index as (1/D) = 1/ pi2, in which, pi is for the relative abundance of each readings and ln is for the natural log. Comparison of compost control efficacy versus the pathogens, metabolic AWCD index, microbial populations, and fluorescein diacetate hydrolysis (FDAH) between the treatments were done by one-way analysis of variance (ANOVA) and means were separated by Tukey's honestly significant difference (HSD) test at p ≤ 0.05. Differences in catabolic properties among microbial communities on Biolog substrates were determined by the principal component analysis (PCA). PCA was also performed on the descriptive catabolic and t-RFs diversity indexes, in order to evaluate differences among suppressiveness of composts along the first and third principal components (PC1 and PC3) presented, chosen for the effectiveness of separating the samples. T-RFLP data were square root transformed and similarity matrices were constructed using the Bray-Curtis similarity distance, which is not affected by the number of null values between samples releasing information on Beta-diversity between community compositions for all biological samples [17]. The dataset rank order was examined by nonmetric multi-dimensional scaling (nMDS) to ordinate the similarity data following 100 random starts.

Compost Disease Suppressiveness
The in vivo suppressive assays showed differential patterns of disease control among composts. Nine out of thirteen raw composts counteracted Rhizoctonia pathogenesis as indicated by damping-off control efficacy in the range of 36-57%, on average. The remaining composts had null effects on disease occurrence. For S. minor eight out of thirteen raw composts showed significant control effects between 60 and 95%, on average ( Figure 1). The groups composed of C1, C3, C4, C8, C11, C12 and C13, and that with C2, C5 and C6 displayed, respectively, the statistically highest (p ≤ 0.05) and lowest biocontrol responses on both pathosystems. Conversely, C7 and C9 were suppressive against Rhizoctonia damping-off only (Figure 1). The net contribution of the biological component of the organic matter in suppressiveness were also measured by comparing raw responses with those of the heated-composts. In general, compost sterilization strongly reduced suppressive properties as shown for seven and nine out of thirteen composts for Rhizoctonia and Sclerotinia damping-off, respectively; providing indications on the specific contribution of biological components on the defined suppressive properties. Surprisingly, C2 increased suppressiveness after sterilization. All on-farm composts showed a significantly (p ≤ 0.05) higher percentage of cress germination index than the toxicity threshold value of 80%, indicating the maturity of the compost [18]; C13 had the highest GI% in comparison with the others ( Figure A1 in Appendix A).

Compost Microbiological Features
The microbial community profiles of the composts are displayed in Figure 2. Population levels of total bacteria ranged between 7.24 and 13.61 Log CFU g −1 , while that of fungi were in the interval 3.21-7.61 Log CFU g −1 (Figure 2A,B). FDAH activity was largest for C5 and C10 followed, in the rank order, by C8, C2 and C13 ( Figure 2C). In Figure 3, the population levels of Bacillus-like and Pseudomonas-like groups are also displayed. Based on the kinetics of color development in Biolog Eco microplates, the average well-color development (AWCD) was highest in C11 followed, in the order, by groups with comparable levels composed of C1 and C12; C5, C6 and C10; C3 and C8; and the remaining lower samples ( Figure 2D). for seven and nine out of thirteen composts for Rhizoctonia and Sclerotinia damping-off, respectively; providing indications on the specific contribution of biological components on the defined suppressive properties. Surprisingly, C2 increased suppressiveness after sterilization. All on-farm composts showed a significantly (p ≤ 0.05) higher percentage of cress germination index than the toxicity threshold value of 80%, indicating the maturity of the compost [18]; C13 had the highest GI% in comparison with the others ( Figure

Compost Microbiological Features
The microbial community profiles of the composts are displayed in Figure. 2. Population levels of total bacteria ranged between 7.24 and 13.61 Log CFU g −1 , while that of fungi were in the interval 3.21-7.61 Log CFU g −1 ( Figure. 2A and B). FDAH activity was largest for C5 and C10 followed, in the rank order, by C8, C2 and C13 ( Figure. 2C). In Figure. 3, the population levels of Bacillus-like and Pseudomonas-like groups are also displayed. Based on the kinetics of color development in Biolog Eco microplates, the average well-color development (AWCD) was highest in C11 followed, in the order, by groups with comparable levels composed of C1 and C12; C5, C6 and C10; C3 and C8; and the remaining lower samples ( Figure. 2D).  farm green composts (C1-13). Bars indicate the mean ± standard deviation. Different letters indicate significant differences according Tukey's HSD test at p ≤ 0.05.

Microbial Biodiversity
Principal component analysis of Biolog catabolic activity revealed the highest levels of compost clustering according to the first and the third components, that account together for 42.48% of the Biolog data variability ( Figure. 4). PC1 and PC3 have been chosen for the better PC1, explaining 26.34% of variation, and was correlated (R > 0.60) positively with the degree of utilization of tween 40, α-cyclodextrin, d-xylose, 2-hydroxy benzoic acid, γ-hydroxybutyric acid, α-ketobutyric acid and glycyl-l-glutamic acid; and negatively with β-methyl-d-glucoside. PC3, accounting for 16.14% of variation, was positively correlated (R > 0.60) with the kinetics of color development on pyruvic acid methyl ester and d-mannitol and negatively with that on putrescine. Ordination biplot in the plane showed that compost communities, on the basis of their Csource utilization capability, form one distinct core group including C1, C4, C5, C8, C10, C11, C12 and C13; whereas composts C3, C6, C7 and C9 are positioned in the first quadrant and C2 is further away, in the third one.
In order to visualize the dissimilarities in community composition between the samples, as a measure of beta-diversity, nMDS plots were calculated based on the Bray-Curtis dissimilarity index. Overall, nMDS revealed two clearly distinct groups: the most numerous one including the samples C1, C2, C3, C4, C6, C7, C8 and C13; and those with C10, C11 and C12 that grouped together. Then, there was a tendency for the samples C5 and C9 to position together in the middle of the plot, distinguished from the other samples ( Figure. 5).

Microbial Biodiversity
Principal component analysis of Biolog catabolic activity revealed the highest levels of compost clustering according to the first and the third components, that account together for 42.48% of the Biolog data variability (Figure 4). PC1 and PC3 have been chosen for the better PC1, explaining 26.34% of variation, and was correlated (R > 0.60) positively with the degree of utilization of tween 40, α-cyclodextrin, d-xylose, 2-hydroxy benzoic acid, γ-hydroxybutyric acid, α-ketobutyric acid and glycyl-l-glutamic acid; and negatively with β-methyl-d-glucoside.
Agriculture 2020, 10, x FOR PEER REVIEW 6 of 11 farm green composts (C1-13). Bars indicate the mean ± standard deviation. Different letters indicate significant differences according Tukey's HSD test at p ≤ 0.05.

Microbial Biodiversity
Principal component analysis of Biolog catabolic activity revealed the highest levels of compost clustering according to the first and the third components, that account together for 42.48% of the Biolog data variability ( Figure. 4). PC1 and PC3 have been chosen for the better PC1, explaining 26.34% of variation, and was correlated (R > 0.60) positively with the degree of utilization of tween 40, α-cyclodextrin, d-xylose, 2-hydroxy benzoic acid, γ-hydroxybutyric acid, α-ketobutyric acid and glycyl-l-glutamic acid; and negatively with β-methyl-d-glucoside. PC3, accounting for 16.14% of variation, was positively correlated (R > 0.60) with the kinetics of color development on pyruvic acid methyl ester and d-mannitol and negatively with that on putrescine. Ordination biplot in the plane showed that compost communities, on the basis of their Csource utilization capability, form one distinct core group including C1, C4, C5, C8, C10, C11, C12 and C13; whereas composts C3, C6, C7 and C9 are positioned in the first quadrant and C2 is further away, in the third one.
In order to visualize the dissimilarities in community composition between the samples, as a measure of beta-diversity, nMDS plots were calculated based on the Bray-Curtis dissimilarity index. Overall, nMDS revealed two clearly distinct groups: the most numerous one including the samples C1, C2, C3, C4, C6, C7, C8 and C13; and those with C10, C11 and C12 that grouped together. Then, there was a tendency for the samples C5 and C9 to position together in the middle of the plot, distinguished from the other samples ( Figure. 5). PC3, accounting for 16.14% of variation, was positively correlated (R > 0.60) with the kinetics of color development on pyruvic acid methyl ester and d-mannitol and negatively with that on putrescine. Ordination biplot in the plane showed that compost communities, on the basis of their C-source utilization capability, form one distinct core group including C1, C4, C5, C8, C10, C11, C12 and C13; whereas composts C3, C6, C7 and C9 are positioned in the first quadrant and C2 is further away, in the third one.
In order to visualize the dissimilarities in community composition between the samples, as a measure of beta-diversity, nMDS plots were calculated based on the Bray-Curtis dissimilarity index. Overall, nMDS revealed two clearly distinct groups: the most numerous one including the samples C1, C2, C3, C4, C6, C7, C8 and C13; and those with C10, C11 and C12 that grouped together. Then, there was a tendency for the samples C5 and C9 to position together in the middle of the plot, distinguished from the other samples ( Figure 5). Multivariate correlation analysis by PCA, shows a biplot distribution of the variables, such as alpha-diversity indices and suppressive levels, along the two components PC1 and PC3, that may be explained by diversity among samples for 41.83% and their suppressive properties for 15.14% of the total variability ( Figure. 6). Moreover, plane ordination indicated that the functional diversity is most associated with the suppressiveness than the genetic diversity.

Discussion
Composts are colonized by a wide spectrum of Procarya and Eukarya microorganisms involved in the breakdown of organic materials on the piles; some of them may potentially play a role in disease suppression [19]. The green waste composts investigated in our study, which varied based on the plant raw material input, showed a differential pattern of suppressiveness over the two assayed pathosystems. Interestingly, suppressiveness was found among compost characterized by a multiple feedstock composition or originated from fennel and artichoke residues, or walnut husk according to previous investigations [8]. Except for C2, sterilization generally diminished or annulled the damping-off control efficacy of these composts, suggesting the decisive role of the microbial biomass in delivering the observed biocontrol functions [20]. C2 is produced by maize and livestock Multivariate correlation analysis by PCA, shows a biplot distribution of the variables, such as alpha-diversity indices and suppressive levels, along the two components PC1 and PC3, that may be explained by diversity among samples for 41.83% and their suppressive properties for 15.14% of the total variability ( Figure 6). Moreover, plane ordination indicated that the functional diversity is most associated with the suppressiveness than the genetic diversity. Multivariate correlation analysis by PCA, shows a biplot distribution of the variables, such as alpha-diversity indices and suppressive levels, along the two components PC1 and PC3, that may be explained by diversity among samples for 41.83% and their suppressive properties for 15.14% of the total variability ( Figure. 6). Moreover, plane ordination indicated that the functional diversity is most associated with the suppressiveness than the genetic diversity.

Discussion
Composts are colonized by a wide spectrum of Procarya and Eukarya microorganisms involved in the breakdown of organic materials on the piles; some of them may potentially play a role in disease suppression [19]. The green waste composts investigated in our study, which varied based on the plant raw material input, showed a differential pattern of suppressiveness over the two assayed pathosystems. Interestingly, suppressiveness was found among compost characterized by a multiple feedstock composition or originated from fennel and artichoke residues, or walnut husk according to previous investigations [8]. Except for C2, sterilization generally diminished or annulled the damping-off control efficacy of these composts, suggesting the decisive role of the microbial biomass in delivering the observed biocontrol functions [20]. C2 is produced by maize and livestock

Discussion
Composts are colonized by a wide spectrum of Procarya and Eukarya microorganisms involved in the breakdown of organic materials on the piles; some of them may potentially play a role in disease suppression [19]. The green waste composts investigated in our study, which varied based on the plant raw material input, showed a differential pattern of suppressiveness over the two assayed pathosystems. Interestingly, suppressiveness was found among compost characterized by a multiple feedstock composition or originated from fennel and artichoke residues, or walnut husk according to previous investigations [8]. Except for C2, sterilization generally diminished or annulled the damping-off control efficacy of these composts, suggesting the decisive role of the microbial biomass in delivering the observed biocontrol functions [20]. C2 is produced by maize and livestock wastes, which could release amounts of ammonia in addition to other fungistatic substances after heating treatment.
In the current work, microbial population and related activities were not predictors of the recorded suppressiveness, since measures of fungal and bacterial densities, as well as catabolic and enzyme functionalities did not correlate with the compost control efficacy on cress disease. However, some indicative information about antagonistic potentialities of composts may be provided by counts of Bacillusand Pseudomonas-like groups. On the other hand, alpha-diversity based on differences in microbial C-source metabolizing ability was associated with compost ability to reduce damping-off more than the descriptive diversity indexes calculated on T-RFLPs targeting 16S rRNA gene, as revealed by PCA. Differences in the suppressive potential of composts can be better predicted by the variations in the community levels of physiological profiles. Catabolic-based functions of the resident communities may be used to discriminate suppressive from nonsuppressive composts [9]. Here, PCA separation of compost samples on the basis of the microbial metabolism, evaluated by the Biolog ® EcoPlate™ system, showed one distinct core cluster including the most suppressive composts.
T-RFLP analysis has been used here to characterize a diversity of 16S rDNA genes relying on variation in the position of restriction sites among sequences. The beta-diversity described by nMDS analysis of the Bray-Curtis dissimilarity separated the different compost samples into more or less distinct functionally meaningful clusters. Alpha-and beta-diversity of microbial communities indicate the relative differences in the composition of species, respectively, within a single habitat and between habitats [21]. In a compost, these parameters are critically influenced by the conditions occurring during the composting process [22][23][24], in which microbial communities likely follow a stochastic assembly model [25].
T-RFLP and Biolog Eco Plate are both accepted methods for assessing the complexity of microbial communities in telluric environments [15,26]. However, the Biolog ® EcoPlate™ classifies the composts very differently than the T-RFLP pattern and better predicts disease suppression. In the current study a proper involvement of specific healthy microbial community structures [27] in defining disease-suppressive properties of the examined on-farm composts has been revealed. Accordingly, in a previous report, Metha et al. [28] categorized specific selective bacterial structures into compost communities associated with suppressiveness against the Fusarium wilt of tomato.
As a rule, compost suppressiveness follows a general model when it is due to the antagonistic action of the totality of the resident microorganisms, whereas a more specific one when it is based on the effects of individual(s) or select groups [29]. Regulating mechanisms of our suppressive composts resemble the latter.
Microbial diversity may have a crucial role in sustaining ecosystem functions and maintaining the natural suppressive activities via compost amendment [30]. Compost microflora were found to be critical in protecting cucumber seedlings from Fusarium oxysporum f.sp. radicis-cucumerinum [31]. In agroindustrial waste composts, it was linked to the control of the Phytophthora root rot on pepper [32] or the muskmelon fusariosis and Phytophthora blight on pepper [33]. However, the composition and the redundant metabolic functions can dramatically affect these properties. Metabarcoding and GeoChip techniques have been applied to composted almond shell-amended soil samples, revealing an enhancement of specific microbial populations implied in the management of avocado white root rot [34]. De Corato et al. [35] recently demonstrated the transferability of suppressiveness in tailor-made composts by sourcing some potential biocontrol groups from high suppressive green compost extracts. This study agrees with previous reports that found the green feedstock such as cropping residues were able to stimulate the formation of very complex microbial structures during the composting process, more so than the agroindustrial byproducts (i.e., leftovers, production scraps, etc.), involved the specific suppression of a significant group of telluric diseases [36,37].

Conclusions
Disease suppressive properties of the green composts obtained through the on-farm composting system, constitute an added value of these sustainable products. Levels of physiological profiles, assessed by elaborating communities' ability to consume the BIOLOG Ecoplate differential panel of