Wound Dressings Coated with Silver Nanoparticles and Essential Oils for The Management of Wound Infections

Infection represents one of the major risk factors in persistent and difficult to treat wounds. This study focuses on developing antimicrobial wound dressings coated with silver nanoparticles, sodium alginate and different essential oils, to avoid wound infection and biofilm formation. The design of the wound dressings was done by the dip coating method. The characteristics of the developed materials were analysed by physicochemical (FT-IR, XRD, SEM, TEM) and biological (antimicrobial tests) approaches. The results demonstrated uniform silver nanoparticle formation on the substrate, and the developed nanomodified dressings were proven to have increased antimicrobial and antibiofilm potential. The developed wound dressings based on silver nanoparticles, sodium alginate and essential oils have real potential in treating infections, and can be investigated as an efficient alternative to antibiotics and topical preparations for wound management.


Introduction
Infection represents one of the main risk factors in wound healing. When the skin function based on the barrier activity against bacterial invasion is lost, a large amount of exudate emerges at the affected site, which, together with the body temperature, provides an ideal place for bacterial growth. [1][2][3][4][5][6].
The most common Gram-positive bacteria that can appear at wound sites is Staphylococcus aureus, which can cause a wide range of infections. These bacteria can lead to significant infections, from the localized affected skin to life-threatening circumstances such as bacteraemia and even pneumonia. Another concern refers to the ability of this opportunistic pathogen to cause hospital-acquired and antibiotic-resistant infections. In many countries, the management of Methicillin-resistant S. aureus (MRSA) represents a difficult task in health care facilities [7][8][9][10][11]. Along with Staphylococcus species, Gram-negative bacteria (such as Escherichia coli and Pseudomonas aeruginosa), but also yeasts (ex. Candida albicans) could colonize wounds and produce persistent infections [12]. A major concern in infected wounds is the formation of biofilms, which are multicellular communities of microbial species

Synthesis of Silver Nanoparticles
For the synthesis of silver nanoparticles, two solutions were prepared: one obtained by dissolving 0.5 g of silver nitrate (AgNO 3 ) in 100 mL of distilled water, and the other, the reductant, by dissolving 1 g of D-glucose and 4 g of sodium hydroxide (NaOH) in 100 mL of distilled water, under continuous stirring at 80 • C. After the preparation of the two solutions, they were mixed by dripping the reductant solution into the silver nitrate solution, a process that modified the colour. The final product was homogenous in nature and was centrifuged, washed, dried for 48 h and mortared for further analysis [35].

Synthesis of The Modified Wound Dressings
Traditional textile wound dressings were purchased from a local supplier and sectioned in four lots of 14 parts, with a size of 1 cm × 1 cm. In order to obtain the desired wound dressings, we chose to make four solutions (one of silver nanoparticles used as a control, and three other silver nanoparticles solutions, each of which was functionalized with different essential oils, i.e., mandarin, niaouli and clove).
To improve the coverage and properties of the dressings, we chose to make a sodium alginate solution by dissolving 1 g alginate (Alg) in 100 mL of distilled water under continuous stirring until homogenization occurred.
The control solution was obtained using the same method as the one mentioned above. After that, one lot of wound dressings was introduced in the silver nanoparticles solution under magnetic stirring for two minutes, until the nanoparticles were incorporated in the wound dressing. Then, the wound dressings were removed from the solution and dropped in the sodium alginate solution for two minutes. Finally, the wound dressings were removed from the sodium alginate solution and deposited on a glass support to dry.
The solutions functionalized with essential oils were obtained using the same method, with the only difference being that in the reductant solution, we added 500 µL of each of the three essential oils. Four lots of wound dressings were produced, including one lot of wound dressings with silver nanoparticles and alginate layer, a second lot with silver nanoparticles plus mandarin oil and alginate layer, a third with silver nanoparticles plus niaouli oil and alginate layer, and a fourth with silver nanoparticles plus clove oil and alginate layer.

Physico-Chemical Characterization
X-ray diffraction (XRD) studies were carried out using a Malvern PANalytical Empyrean (Almelo, The Netherlands) equipped with a hybrid monochromator 2 x Ge 220 on the incident side and a parallel plate collimator mounted on the PIXcel 3D detector on the diffracted side. Grazing Incidence X-ray Diffraction (GIXRD) measurements were performed at room temperature, with an incidence angle ω = 0.5 • for Bragg angle values of 2θ between 10 • and 80 • , using Cu Kα radiation with λ = 1.5406 Å (40 mA and 45 kV).
The morphology of the samples was analysed using a Quanta Inspect F FEG (field emission gun) scanning electron microscope (SEM) with 1.2 nm resolution equipped with an energy-dispersive X-ray (EDX) analyzer with a resolution of 133 eV at MnKα (Thermo Fisher (former FEI) (Hillsboro, OR, USA).
The high-resolution transmission electron microscope (TEM) images of the samples were obtained on finely powdered samples using a 80-200 KV Titan Themis high-resolution TEM from (Thermo Fisher (former FEI) (Hillsboro, OR, USA). The microscope was operated in transmission mode at 200 kV.

Antimicrobial Evaluation
Antimicrobial tests were performed in vitro by using three clinically relevant and model microbial species: Gram-negative (Escherichia coli ATCC 25922, Gram-positive: Staphylococcus aureus ATCC 23235 and the yeast strain Candida albicans ATCC 10231. The antimicrobial properties of the developed materials were analysed by qualitative (i.e., evaluation of growth inhibition zone) and quantitative methods (i.e., growth of planktonic and attached cultures). For the evaluation of growth, the inhibition zone fragments (1 cm × 1 cm) of the developed materials were sterilized by UV exposure for 30 min. Sterile materials (from each sample) were aseptically placed on nutritive agar plates previously inoculated with 0.5 McFarland (1.5 × 10 8 colony forming units/mL) microbial suspensions prepared from the tested microbial strains. The inoculation of microbial strains was performed according to the disc-diffusion antibiogram assay using a sterile swab. The inoculated Petri dishes containing the tested materials were incubated at 37 • C for 24 h to allow microbial development. After incubation, growth Materials 2020, 13, 1682 4 of 13 inhibition was evaluated. After the incubation time, the growth inhibition zone developed around and under the materials was measured.
To assess the impact of the coatings on microbial growth in both free-floating (planktonic) and attached cultures, selected microbial strains were grown in nutritive broth in the presence of the coatings. One fragment of sterile material from each sample was placed in a six-well sterile plate. Then, 2 mL of nutritive broth was added into the plates and they were inoculated with 20 µL each of the prepared microbial 0.5 McFarland suspensions. These plates were incubated for 24 h at 37 • C to allow microbial growth and biofilm formation. After incubation, the absorbance of planktonic cultures developed in the presence of the coatings was measured using a spectrophotometer at a set absorbance of 600 nm.
In order to assess the ability of the microbial cells to attach and develop biofilms after 24 h of incubation, the wound dressings from the previously prepared six-well plates were washed with sterile saline water and introduced in sterile Eppendorf tubes in 1 mL sterile saline water. The washed coatings containing attached cells were further vigorously vortexed to detach the attached cells, and then the resulting suspensions were diluted and inoculated on agar plates to perform viable count analysis and calculate colony forming units (CFU)/mL values. Statistical significance was analysed by Student's T-test. Values of P lower than 0.05 were considered statistically significant.

Silver Nanoparticles Characterization
3.1.1. X-ray Diffraction (XRD) Figure 1 represents the XRD diffractogram obtained on the silver nanoparticles powder, where the crystallinity and the phase of interest are analysed.
Materials 2020, 13, x FOR PEER REVIEW 4 of 13 development. After incubation, growth inhibition was evaluated. After the incubation time, the growth inhibition zone developed around and under the materials was measured.
To assess the impact of the coatings on microbial growth in both free-floating (planktonic) and attached cultures, selected microbial strains were grown in nutritive broth in the presence of the coatings. One fragment of sterile material from each sample was placed in a six-well sterile plate. Then, 2 mL of nutritive broth was added into the plates and they were inoculated with 20 μL each of the prepared microbial 0.5 McFarland suspensions. These plates were incubated for 24 h at 37 °C to allow microbial growth and biofilm formation. After incubation, the absorbance of planktonic cultures developed in the presence of the coatings was measured using a spectrophotometer at a set absorbance of 600 nm.
In order to assess the ability of the microbial cells to attach and develop biofilms after 24 h of incubation, the wound dressings from the previously prepared six-well plates were washed with sterile saline water and introduced in sterile Eppendorf tubes in 1 mL sterile saline water. The washed coatings containing attached cells were further vigorously vortexed to detach the attached cells, and then the resulting suspensions were diluted and inoculated on agar plates to perform viable count analysis and calculate colony forming units (CFU)/mL values. Statistical significance was analysed by Student's T-test. Values of P lower than 0.05 were considered statistically significant.

Silver Nanoparticles Characterization
3.1.1. X-ray Diffraction (XRD) Figure 1 represents the XRD diffractogram obtained on the silver nanoparticles powder, where the crystallinity and the phase of interest are analysed. It was observed that the interferences of the powder corresponded to pure phase cubic silver. The spectra presented diffraction peaks for the defined Miller indices planes (111), (200), (220), (311), in accordance with the American Society for Testing and Materials (ASTM) sheet for silver (ICDD PDF4+ 00-004-0783). The degree of crystallinity of the sample is evident by the intensity of the diffraction lines. The crystallite size was also calculated using the Scherrer formula; the obtained average crystallite size was 21.60 nm. It was observed that the interferences of the powder corresponded to pure phase cubic silver. The spectra presented diffraction peaks for the defined Miller indices planes (111), (200), (220), (311), in accordance with the American Society for Testing and Materials (ASTM) sheet for silver (ICDD PDF4+ 00-004-0783). The degree of crystallinity of the sample is evident by the intensity of the diffraction lines. The crystallite size was also calculated using the Scherrer formula; the obtained average crystallite size was 21.60 nm.

Scanning Electron Microscopy (SEM)
In order to investigate the sample morphology and the particles sizes, a SEM analysis was performed. In Figure 2, the SEM images are presented for the silver nanoparticle powder and the measured particle size distribution.
Materials 2020, 13, x FOR PEER REVIEW 5 of 13 In order to investigate the sample morphology and the particles sizes, a SEM analysis was performed. In Figure 2, the SEM images are presented for the silver nanoparticle powder and the measured particle size distribution. In Figure 2, the structure and size of the nanoparticles are analysed. The nanoparticles are in agglomerated powder. The particles presented almost spherical shapes, which are known for their antimicrobial activity. As seen in the particle size distribution graph, the mean particle size was 63 +/-2 nm. The nanoparticles were also distributed bimodally with a maxima at around 55 nm and 85 nm.

Transmission Electron Microscopy (TEM)
To better observe the morphology and crystallinity of the nanoparticles, a TEM analysis was performed. The images are shown in Figure 3.  In Figure 2, the structure and size of the nanoparticles are analysed. The nanoparticles are in agglomerated powder. The particles presented almost spherical shapes, which are known for their antimicrobial activity. As seen in the particle size distribution graph, the mean particle size was 63 +/− 2 nm. The nanoparticles were also distributed bimodally with a maxima at around 55 nm and 85 nm.

Transmission Electron Microscopy (TEM)
To better observe the morphology and crystallinity of the nanoparticles, a TEM analysis was performed. The images are shown in Figure 3. In order to investigate the sample morphology and the particles sizes, a SEM analysis was performed. In Figure 2, the SEM images are presented for the silver nanoparticle powder and the measured particle size distribution. In Figure 2, the structure and size of the nanoparticles are analysed. The nanoparticles are in agglomerated powder. The particles presented almost spherical shapes, which are known for their antimicrobial activity. As seen in the particle size distribution graph, the mean particle size was 63 +/-2 nm. The nanoparticles were also distributed bimodally with a maxima at around 55 nm and 85 nm.

Transmission Electron Microscopy (TEM)
To better observe the morphology and crystallinity of the nanoparticles, a TEM analysis was performed. The images are shown in Figure 3.  TEM images for the silver nanoparticle powder reveal that the particles are composed of soft agglomerates with a connection between all the independent particles. The morphology of the nanoparticles is characterized by their polyhedral shape. The average particle is 69 +/− 2 nm. The crystallite size calculated from XRD analysis (21.603 nm) highlights the polycrystalline nature of the particles.

Scanning Electron Microscopy (SEM)
To evaluate the distribution of the silver nanoparticles in the commercial wound dressing, the samples with nanoparticles, sodium alginate and different essential oils were analysed with the SEM equipment. In the Figures below SEM images of the obtained modified commercial dressings are shown.
The images obtained on the wound dressing covered with sodium alginate and dipped into the Ag nanoparticle solution show that the coating of the dressing with nanoparticles formed from nanoparticle agglomerates and some individual distributed nanoparticles, as shown in Figure 4. nanoparticles is characterized by their polyhedral shape. The average particle is 69 +/-2 nm. The crystallite size calculated from XRD analysis (21.603 nm) highlights the polycrystalline nature of the particles.

Scanning Electron Microscopy (SEM)
To evaluate the distribution of the silver nanoparticles in the commercial wound dressing, the samples with nanoparticles, sodium alginate and different essential oils were analysed with the SEM equipment. In the Figures below SEM images of the obtained modified commercial dressings are shown.
The images obtained on the wound dressing covered with sodium alginate and dipped into the Ag nanoparticle solution show that the coating of the dressing with nanoparticles formed from nanoparticle agglomerates and some individual distributed nanoparticles, as shown in Figure 4.
For the samples also coated with mandarin oil, we can see that the oil itself has a big influence in nanoparticle distribution on the surface of the dressing ( Figure 5). Thus, the nanoparticles are still present in agglomerated form but with smaller dimensions and with improved coverage on the surface of the fibers, as well as being more homogenous. Figure 6 shows the SEM images of the wound dressing with silver nanoparticles and niaouli oil. For these samples, the addition of the essential oil resluted in improved formation and distribution of the silver nanoparticles. Moreover, the oil properties influence the nanoparticles' behaviour so that they adhere to the dressing fibers.  For the samples also coated with mandarin oil, we can see that the oil itself has a big influence in nanoparticle distribution on the surface of the dressing ( Figure 5). Thus, the nanoparticles are still present in agglomerated form but with smaller dimensions and with improved coverage on the surface of the fibers, as well as being more homogenous.  In Figure 7, the SEM images for the fourth sample which contain clove oil are shown. In the SEM images, the commercial wound dressings with silver nanoparticles, alginate and clove oil show a clear and obvious accumulation of silver nanoparticles on the surface and between the dressing fibers. Moreover, the nanoparticles are more homogenous and significant in quantity compared to the other samples. The clove oil exhibits best improves the distribution of the silver nanoparticles on the modified dressing.  Figure 6 shows the SEM images of the wound dressing with silver nanoparticles and niaouli oil. For these samples, the addition of the essential oil resluted in improved formation and distribution of the silver nanoparticles. Moreover, the oil properties influence the nanoparticles' behaviour so that they adhere to the dressing fibers.  In Figure 7, the SEM images for the fourth sample which contain clove oil are shown. In the SEM images, the commercial wound dressings with silver nanoparticles, alginate and clove oil show a clear and obvious accumulation of silver nanoparticles on the surface and between the dressing fibers. Moreover, the nanoparticles are more homogenous and significant in quantity compared to the other samples. The clove oil exhibits best improves the distribution of the silver nanoparticles on the modified dressing. In Figure 7, the SEM images for the fourth sample which contain clove oil are shown. In the SEM images, the commercial wound dressings with silver nanoparticles, alginate and clove oil show a clear and obvious accumulation of silver nanoparticles on the surface and between the dressing fibers. Moreover, the nanoparticles are more homogenous and significant in quantity compared to the other samples. The clove oil exhibits best improves the distribution of the silver nanoparticles on the modified dressing.

Antimicrobial Activity-Evaluation of Growth Inhibition Zone
Our data revealed that after 24 h incubation at 37 °C , all the used materials exhibited diverse inhibition zones, with diameters ranging from 14-20 mm for S. aureus, 14-18 mm for E. coli and 30-39 mm for Candida albicans (Figure 8). The highest inhibition zone was observed for the C. albicans tested strain, where zone inhibition reached 39 mm for dressings coated with mandarin essential oil (EO) functionalized silver nanoparticles (NPs). In all the tested strains, the coatings containing nanoparticles functionalized with mandarin and clove EOs manifested the highest antimicrobial impact (Figure 8).

Antimicrobial Activity-Growth of Planktonic and Attached Microbial Cultures
The growth of planktonic culture was different in the presence of the designed nanocoatings, depending on the utilized essential oil and the tested microbial cells. In S. aureus, the most significant growth inhibition was achieved in the presence of cloves and mandarin EOs functionalized silver nanoparticles ( Figure 9). However, niaouli EO functionalized silver nanoparticles (NPs) also exhibited significant inhibition of planktonic culture growth in S.aureus (i.e., more than four fold).

Antimicrobial Activity-Evaluation of Growth Inhibition Zone
Our data revealed that after 24 h incubation at 37 • C, all the used materials exhibited diverse inhibition zones, with diameters ranging from 14-20 mm for S. aureus, 14-18 mm for E. coli and 30-39 mm for Candida albicans (Figure 8). The highest inhibition zone was observed for the C. albicans tested strain, where zone inhibition reached 39 mm for dressings coated with mandarin essential oil (EO) functionalized silver nanoparticles (NPs). In all the tested strains, the coatings containing nanoparticles functionalized with mandarin and clove EOs manifested the highest antimicrobial impact (Figure 8).

Antimicrobial Activity-Evaluation of Growth Inhibition Zone
Our data revealed that after 24 h incubation at 37 °C , all the used materials exhibited diverse inhibition zones, with diameters ranging from 14-20 mm for S. aureus, 14-18 mm for E. coli and 30-39 mm for Candida albicans (Figure 8). The highest inhibition zone was observed for the C. albicans tested strain, where zone inhibition reached 39 mm for dressings coated with mandarin essential oil (EO) functionalized silver nanoparticles (NPs). In all the tested strains, the coatings containing nanoparticles functionalized with mandarin and clove EOs manifested the highest antimicrobial impact (Figure 8).

Antimicrobial Activity-Growth of Planktonic and Attached Microbial Cultures
The growth of planktonic culture was different in the presence of the designed nanocoatings, depending on the utilized essential oil and the tested microbial cells. In S. aureus, the most significant growth inhibition was achieved in the presence of cloves and mandarin EOs functionalized silver nanoparticles ( Figure 9). However, niaouli EO functionalized silver nanoparticles (NPs) also exhibited significant inhibition of planktonic culture growth in S.aureus (i.e., more than four fold).

Antimicrobial Activity-Growth of Planktonic and Attached Microbial Cultures
The growth of planktonic culture was different in the presence of the designed nanocoatings, depending on the utilized essential oil and the tested microbial cells. In S. aureus, the most significant growth inhibition was achieved in the presence of cloves and mandarin EOs functionalized silver nanoparticles ( Figure 9). However, niaouli EO functionalized silver nanoparticles (NPs) also exhibited significant inhibition of planktonic culture growth in S.aureus (i.e., more than four fold). In the case of E. coli, the most significant growth inhibition was achieved in the presence of mandarin EO functionalized silver NPs, with the values obtained for niaouli and clove EOs being similar to those obtained for the plain silver NPs (Figure 10). In the case of E. coli, the most significant growth inhibition was achieved in the presence of mandarin EO functionalized silver NPs, with the values obtained for niaouli and clove EOs being similar to those obtained for the plain silver NPs (Figure 10). In the case of E. coli, the most significant growth inhibition was achieved in the presence of mandarin EO functionalized silver NPs, with the values obtained for niaouli and clove EOs being similar to those obtained for the plain silver NPs (Figure 10).  In the case of C. albicans, the most significant growth inhibition was achieved in the presence of clove EO functionalized silver NPs. However, mandarin and niaouli EOs functionalized NPs also manifested a high inhibition of planktonic cultures ( Figure 11).
Materials 2020, 13, x FOR PEER REVIEW 10 of 13 dressing modified with silver NPs and niaouli EO, Clove oil Dressing-dressing modified with silver NPs and clove EO. *P < 0.05 (control coatings vs. NPs and EOs coated samples for each strain).
In the case of C. albicans, the most significant growth inhibition was achieved in the presence of clove EO functionalized silver NPs. However, mandarin and niaouli EOs functionalized NPs also manifested a high inhibition of planktonic cultures ( Figure 11). Figure 11. Growth of planktonic cultures of C. albicans in the presence of wound dressings coated with EOs functionalized silver nanoparticles (NPs). Coatings Control-dressing modified with silver NPs, Mandarin oil Dressing-dressing modified with silver NPs and mandarin EO, Niaouli oil Dressingdressing modified with silver NPs and niaouli EO, Cloves oil Dressing-dressing modified with silver NPs and clove EO. *P < 0.05 (control coatings vs. NPs and EOs coated samples for each strain).
In order to evaluate the ability of microbial cells to develop biofilms on the developed materials, cells were incubated for 24 h in the presence of S. aureus, E.coli and C. albicans, after which the correlated results were recorded, as shown in the graph below. The results demonstrated that all the tested nanocoated wound dressings had an inhibitory effect against microbial attachment by S. aureus. Compared with the nanoparticle free control, the most significant attachment inhibition was observed in the case of clove and niaouli nanoparticle coatings ( Figure 12). All EO functionalized silver nanoparticle coated wound dressings have an enhanced antibiofilm effect, especially the niaouli containing dressing, on the Gram-negative, E. coli and the yeast tested strain, C. albicans. This suggests that niaouli EO is more efficient against microbial attachment and biofilm formation, while mandarin and clove EOs are more efficient at diminishing microbial growth in planktonic, free floating cells.
Moreover, from the SEM images obtained for the designed wound dressings, it can be seen that the clove oil influences the distribution of silver nanoparticles. A correlation can be made between the presence of clove oil along with the abundance of silver NPs on the dressing and the results after the antimicrobial activity. We can say that there is a synergistic effect between clove oil and silver nanoparticles, as highlighted by the evaluation of the growth inhibition zone and growth of planktonic cells, where clove oil was the most effective, compared to the others used. Overall, the SEM results show that the presence of niaouli oil improves the silver nanoparticles distribution on the commercial dressing. In this sense, the effect of niaouli oil dressing against biofilm formation is increased. Figure 11. Growth of planktonic cultures of C. albicans in the presence of wound dressings coated with EOs functionalized silver nanoparticles (NPs). Coatings Control-dressing modified with silver NPs, Mandarin oil Dressing-dressing modified with silver NPs and mandarin EO, Niaouli oil Dressing-dressing modified with silver NPs and niaouli EO, Cloves oil Dressing-dressing modified with silver NPs and clove EO. * p < 0.05 (control coatings vs. NPs and EOs coated samples for each strain).
In order to evaluate the ability of microbial cells to develop biofilms on the developed materials, cells were incubated for 24 h in the presence of S. aureus, E.coli and C. albicans, after which the correlated results were recorded, as shown in the graph below. The results demonstrated that all the tested nanocoated wound dressings had an inhibitory effect against microbial attachment by S. aureus. Compared with the nanoparticle free control, the most significant attachment inhibition was observed in the case of clove and niaouli nanoparticle coatings ( Figure 12). All EO functionalized silver nanoparticle coated wound dressings have an enhanced antibiofilm effect, especially the niaouli containing dressing, on the Gram-negative, E. coli and the yeast tested strain, C. albicans. This suggests that niaouli EO is more efficient against microbial attachment and biofilm formation, while mandarin and clove EOs are more efficient at diminishing microbial growth in planktonic, free floating cells.
Moreover, from the SEM images obtained for the designed wound dressings, it can be seen that the clove oil influences the distribution of silver nanoparticles. A correlation can be made between the presence of clove oil along with the abundance of silver NPs on the dressing and the results after the antimicrobial activity. We can say that there is a synergistic effect between clove oil and silver nanoparticles, as highlighted by the evaluation of the growth inhibition zone and growth of planktonic cells, where clove oil was the most effective, compared to the others used. Overall, the SEM results show that the presence of niaouli oil improves the silver nanoparticles distribution on the commercial dressing. In this sense, the effect of niaouli oil dressing against biofilm formation is increased.

Conclusions
Natural products such as plant-derived extracts and essential oils could be useful in the therapy and prevention of infectious diseases, especially in the context of increasing antibiotic resistance rates. Moreover, nanotechnology represents an efficient way to improve the activity of such bioactive compounds and potentiate their antimicrobial activity. In this paper, we evaluated the potential of silver NPs functionalized with EOs to inhibit microbial colonization and biofilm formation on nanocoated wound dressings. The use of natural compounds and materials such as alginate and essential oils (mandarin, clove and niaouli) together with silver NPs have permitted the development of improved wound dressings which could be efficiently utilized for the management of wounds, by avoiding infection without the use of antibiotics and antiseptic topic products.

Conclusions
Natural products such as plant-derived extracts and essential oils could be useful in the therapy and prevention of infectious diseases, especially in the context of increasing antibiotic resistance rates. Moreover, nanotechnology represents an efficient way to improve the activity of such bioactive compounds and potentiate their antimicrobial activity. In this paper, we evaluated the potential of silver NPs functionalized with EOs to inhibit microbial colonization and biofilm formation on nanocoated wound dressings. The use of natural compounds and materials such as alginate and essential oils (mandarin, clove and niaouli) together with silver NPs have permitted the development of improved wound dressings which could be efficiently utilized for the management of wounds, by avoiding infection without the use of antibiotics and antiseptic topic products.