Nontoxic Glucomoringin-Isothiocyanate (GMG-ITC) Rich Soluble Extract Induces Apoptosis and Inhibits Proliferation of Human Prostate Adenocarcinoma Cells (PC-3)

The incidence of prostate cancer malignancy along with other cancer types is increasing worldwide, resulting in high mortality rate due to lack of effective medications. Moringa oleifera has been used for the treatment of communicable and non-communicable ailments across tropical countries, yet, little has been documented regarding its effect on prostate cancer. We evaluated the acute toxicity and apoptosis inducing effect of glucomoringin-isothiocyanate rich soluble extracts (GMG-ITC-RSE) from M. oleifera in vivo and in vitro, respectively. Glucomoringin was isolated, identified, and characterized using fundamental analytical chemistry tools where Sprague-Dawley (SD) rats, murine fibroblast (3T3), and human prostate adenocarcinoma cells (PC-3) were used for acute toxicity and bioassays experiments. GMG-ITC-RSE did not instigate adverse toxic reactions to the animals even at high doses (2000 mg/kg body weight) and affected none of the vital organs in the rats. The extract exhibited high levels of safety in 3T3 cells, where more than 90% of the cells appeared viable when treated with the extract in a time-dependent manner even at high dose (250 µg/mL). GMG-ITC-RSE significantly triggered morphological aberrations distinctive to apoptosis observed under microscope. These findings obviously revealed the putative safety of GMG-ITC-RSE in vivo and in vitro, in addition to its anti-proliferative effect on PC-3 cells.


Sample Preparation and Characterization
Dried pods of M. oleifera were chopped and ground into fine powder particles, and the sample extracted using a modified technique described by Vongsak et al. [44]. Briefly, the dried powder was suspended in cold water in a standard ratio of 1:3 solute to solvent, the mixture was vortex and kept at room temperature for 24 h. The water soluble suspension was removed after centrifugation for 10 min at 5000 rpm. The supernatant was further filtered with Whatman filter paper no 1 and concentrated using a rotary evaporator under reduced pressure. The resulting milky colored extract was freeze dried, packaged, and kept at 4 • C for further analysis.

Nuclear Magnetic Resonance (NMR)
Basically, proton, carbon, and 2-dimensional nuclear magnetic resonance ( 1 H, 13 C, and 2D-NMR) analyses were conducted and the spectra of glucomoringin were verified on a 500 MHz Varian MNR system (Agilent Technologies, Santa Clara, CA, USA). Chemical shifts were standardized relative to deuterium oxide (D 2 O) and expressed in parts per million (ppm).

High Performance Liquid Chromatography (HPLC)
GMG was identified on a Thermo Scientific Dionex Ultimate 3000 UHLC System (Dionex, Sunnyvale, CA, USA) equipped with a C18 column (250 mm × 5.0 mm × 5 µM). An isocratic program of 100% milliQ at 1 mL/min flow rate was performed on GMG-ITC-RSE in multiple ways together with sinigrin (Sigma Aldrich, St. Louis, MO, USA) as the internal standard, and chromatograms were documented.

Liquid Chromatography Mass Spectrometry (LC/MS)
LC/MS analysis was carried out on a Thermo Finningan LCQ Fleet Ion Trap Mass Spectrometer (Thermo Fisher Scientific Inc., Waltham, MA, USA) equipped with a C18 reverse phase column. The compounds were separated using an isocratic program of 100% milliQ similar to that of HPLC. The actual mass of GMG was obtained with ESI detection in the positive mode by injecting 200 µL of sample into the mass spectrometer (6550 iFunnel Q-TOF LC/MS, Agilent Technologies, Santa Clara, CA, USA) at 0.1 mL/min flow rate using a mass spec syringe employing 0.1% formic acid in methanol as the mobile phase.

Ethics Statement
The entire animal experiment and sample collections were carried out within the barrier system and a necropsy at Makmal Bioserasi, Center for Research and Instrumentation Management (CRIM), Universiti Kebangsaan Malaysia. The protocols were approved by the University's Animal Ethics Committee (UKMAEC). The study was conducted in compliance with the appropriate provision of the OECD (2002), Test No. 423: Acute Oral Toxicity-Acute Toxic class method, OECD guidelines for testing of Chemicals Section 4, OECD Publishing Paris.

Animal Grouping and Extract Administration
Inbreed specific pathogen free (SPF) female Sprague-Dawley (SD) rats weighing 249.94 g in the test group, 232.36 g in the continuing test group, and 260.18 g in the control group were obtained from the Animal Breeding Center, Faculty of Science Technology, Universiti Kebangsaan Malaysia. The animals were housed in an appropriate polycarbonate cage with solid bottoms and side with a stainless steel lid as recommended in the Animal Husbandry Guidelines. They were acclimatized for six days under 18.3 • C to 24 • C and 12 h light/dark cycle lightening condition prior to treatment, during which a certified rodent diet (Brastock) and filtered tap water were provided ad libitum. The rats were keep at a one per cage density. All rats neither underwent a previous procedure nor had an abnormal clinical condition prior to study. SD rats were divided randomly into three groups including the test group. A 2000 mg/kg body weight of glucomoringin-isothiocyanate rich soluble extract (GMG-ITC-RSE) in 10 mL/kg body weight pre-filtered water was dosed orally into the test and continuing test groups containing four animals each, whereas control animals were given 10 mL/kg body weight pre-filtered water daily in a similar manner for 14 days.

Body Weight Measurement and Observation of Clinical Signs
During the experiment, the weight changes of the animals, which are an important toxicity index for rats, were measured before grouping, dosing, and sacrifice for statistical analysis. Meanwhile, the clinical signs before and after dosing were carefully monitored in such a way that the animals in the test and control groups were observed for morbidity and mortality at 30 min, hourly, and up to four hours, six hours post dosing, and once daily up to 14 days. Additionally, the animals in the test groups were observed at 30 min, hourly up to four hours, six hours post dosing, and at 24 h. The clinical signs were scored according to the features described in Table 1 and the severity scores were recorded in Table 2. Where A-K and a-c refer to the respective main and sub studied clinical features. The digits in column 3 signify the scores for severity of the condition (if any) as defined in Table 2 below, with 0 being a normal/unchanged condition.

Necropsy
A complete necropsy was performed on all the animals under study by examining the external features of the carcass and external orifices. The major internal organs were exposed by dissection, and a detailed gross examination of the cranial, thoracic, and abdominal cavities and their contents was performed. The brain, spleen, liver, heart, pancreas, lungs, kidneys, and stomach were appropriately trimmed of any adherent tissue and weighed individually.

Cell Culture
The human prostate adenocarcinoma cells (PC-3) and murine fibroblast cells (3T3) used in this study were obtained from the American Type Culture Collection. Cells were grown in RPMI-1640 media (Nacalai, Tesque Inc., Kyota, Japan) supplemented with 10% fetal bovine serum (FBS) and a 1% penicillin (10,000 units/mL)-streptomycin (10,000 µg/mL) mixed solution (stabilized), and were maintained at 37 • C in a 5% CO 2 and 95% humidified atmospheric air incubator. The PC-3 are stable and immortalized cancer cells, and experiments were conducted when the cells reached 60-80% confluence with not more than 25 passages.

Cytotoxicity and Anti-Proliferation Examination of GMG-ITC Rich Soluble Extract on PC3 and 3T3 Cells
The GMG-ITC rich soluble extract (GMG-ITC-RSE) was tested for cytotoxic and anti-proliferation activities on the PC-3 and 3T3 cell lines employing the MTT assay as a reliable tool for such analysis as described by Devi and Thangam [45]. Briefly, PC-3 and 3T3 cells were seeded separately in 96-wells culture plates at a density of 1 × 10 5 cells/mL. Following the cells' attachment after 24 h, 1-100 µg/mL of soluble extract and cisplatin were considered as the selected concentrations for the GMG-ITC-RSE and positive control, respectively, whereas 0.1% DMSO was used as the negative control. The cells were incubated in a 5% CO 2 and 95% humidified atmospheric air incubator at 37 • C for 24, 48, and 72 h, respectively. The MTT solution (5 mg/mL) was added to the wells thereafter according to the enclosed instructions and incubated for another four hours to allow for the complete formation of the formazan complex by live cells, followed by the replacement of media with DMSO to dissolve the complex formed. All the experiments were conducted in triplicate, where the optical (OD) density was measured on a micro plate reader (Synergy H1, BioTek, Winooski, USA) at 570 nm as described by Satria et al. [46]. The growth inhibition of the test agent is expressed as the IC 50 value.

Morphological Assessment of GMG-ITC Rich Soluble Extract Treated PC-3 Cells by Inverted Light Microscope
PC-3 cells were seeded in 6-well plates at a density of 2 × 10 5 cells/mL and incubated overnight for proper attachment. The cells were then exposed to a 2.5 µg/mL GMG-ITC rich soluble extract (GMG-ITC-RSE alongside cisplatin (positive control) for 24, 48, and 72 h, respectively. The treated cells were observed at 20× magnification under an inverted light microscope (Axio Vert A1, Carl-Zeiss-Straße, Oberkochen, Germany), equipped with a camera and image acquisition software (AxioCam MRm, Carl-Zeiss-Straße, Oberkochen, Germany). Multiple images were taken independently.

Morphological Assessment of Apoptotic Cells by Acridine Orange and Propidium Iodide (AO/PI) Double Staining
PC-3 cells were seeded in 6-well plates at a density of 2 × 10 5 cells/mL, treated, and incubated as described above. Growth media were removed and discarded when the incubation periods elapsed. Cells were then stained according to the enclosed protocol using 10 µL from the mixture of 10 µg/mL AO and PI each, respectively. The stained cells were assessed using an inverted fluorescence microscope (Axio Vert A1, Carl-Zeiss-Straße, Oberkochen, Germany) equipped with a digital imaging system (AxioCam MRm, Carl-Zeiss-Straße, Oberkochen, Germany) and multiple images were also taken independently.

Statistical Analysis
SPSS statistical software version 22.0 (SPP Inc., Chicago, IL, USA) was used for the analysis and the data presented were taken in triplicate independently. Comparison of means in the acute toxicity study was performed using one way ANOVA employing the Post Hoc feature, whereas the data from the cytotoxic activities of the GMG-ITC rich soluble extract, cisplatin, and 0.1% DMSO on PC-3 cells was performed using the student t-test and p < 0.05 was considered statistically significant.

Chemical Profiling of GMG-ITC Rich Soluble Extract (GMG-ITC-RSE)
NMR analysis was conducted on the GMG-ITC-RSE, and the 1 H-NMR and 13 C-NMR spectra for GMG were observed and the chemical shift data were recorded and presented in Table 3. Numbering of carbon atoms in the table was done according to the individual moieties that made up the structure shown in Figure 1. The conformation of rhamnosyl and that of glucose moieties were confirmed considering the J-couplings of hydrogens attached to position 1 to 5 of the moieties. The 1 H, 13 C and 2D: heteronuclea single quantum coherence (HSQC) and correlation spectroscopy (COSY) NMR analyses provided clear peaks that differentiated the rhamnose to glucose moieties in addition to other peaks typical of aromatic rings, particularly benzene, which signifies the presence of glucosinolates containing sugar moieties. HPLC analysis of GMG-ITC-RSE revealed a peak around 4.940 min (retention time) at 245 nm, similar to that of sinigrin (4.940 min), the internal standard ( Figure 2), as reported by [47], which indicated the presence of glucosinolates in the extract. Identification of the M. oleifera derived glucosinolate under investigation was further corroborated by mass spectrometric examination where LC/MS ESI analyses in the positive mode revealed an m/z 588 (M + H + ) ( Figure 3), which corresponded to the mass of intact GMG ( Figure 1) reported by [48]. The combination of NMR, HPLC, and LC/MS analytical data therefore confirmed the presence of 4-(a-rhamnosyloxy) benzyl glucosinolate, known as GMG, in the extract.

Survival, Observations, and Body Weight of Animal after GMG-ITC Rich Soluble Extract Dosage
After high levels of extract dosage, the survival of animals under investigation and clinical observations were made where all of the animals in the test group, continuing test group, and control group survived the course of the study period with no evidence of either clinical or toxic manifestation as shown in Tables 1 and 2 in the previous section. However, there was no sign of weight loss when the weight of animals in the test and control groups was measured at two intervals (before treatment and termination of the experiment) at the study period as reported in Figure 4.

Survival, Observations, and Body Weight of Animal after GMG-ITC Rich Soluble Extract Dosage
After high levels of extract dosage, the survival of animals under investigation and clinical observations were made where all of the animals in the test group, continuing test group, and control group survived the course of the study period with no evidence of either clinical or toxic manifestation as shown in Tables 1 and 2 in the previous section. However, there was no sign of weight loss when the weight of animals in the test and control groups was measured at two intervals (before treatment and termination of the experiment) at the study period as reported in Figure 4.

Survival, Observations, and Body Weight of Animal after GMG-ITC Rich Soluble Extract Dosage
After high levels of extract dosage, the survival of animals under investigation and clinical observations were made where all of the animals in the test group, continuing test group, and control group survived the course of the study period with no evidence of either clinical or toxic manifestation as shown in Tables 1 and 2 in the previous section. However, there was no sign of weight loss when the weight of animals in the test and control groups was measured at two intervals (before treatment and termination of the experiment) at the study period as reported in Figure 4.  Figure 4. Body weight of animals in the control group and test group measured before the initiation and termination of the study. Results are presented as mean ± SD body weight, while p < 0.05 is considered statistically significant. Means superscripted with the same letter (a) are not different (p > 0.05) statistically unless stated otherwise.

Effect of GMG-ITC Rich Soluble Extract on Animal Organs' Weight
Result of the gross examination of all the animals in the test group and control group revealed no remarkable abnormalities in the investigated organs, which included the brain, kidney lungs, liver, stomach, spleen, heart, and pancreas when subjected to necropsy. Moreover, no significant (p > 0.05) difference in the mean percentage of organ weight to animal body weight between the test group and the control group was observed as shown in Figure 5. The safety of M. oleifera was deeply explained by the accumulated number of studies reported by Stohs and Hartman in [14].

Effect of GMG-ITC Rich Soluble Extract on Animal Organs' Weight
Result of the gross examination of all the animals in the test group and control group revealed no remarkable abnormalities in the investigated organs, which included the brain, kidney lungs, liver, stomach, spleen, heart, and pancreas when subjected to necropsy. Moreover, no significant (p > 0.05) difference in the mean percentage of organ weight to animal body weight between the test group and the control group was observed as shown in Figure 5. The safety of M. oleifera was deeply explained by the accumulated number of studies reported by Stohs and Hartman in [14]. Figure 5. Comparison of organ weight relative to animal body weight in the test and control groups measured during the necropsy experiment. Results are presented as mean ± SD in percentage. While p < 0.05 is considered significant, similar letters on the means in every cluster indicated no significant (p > 0.05) difference between the two means unless stated otherwise.

Cytotoxicity and Anti-Proliferation Evaluation of GMG-ITC Rich Soluble Extract
The MTT result demonstrated an impressive cytotoxic activity of GMG-ITC rich soluble extract (GMG-ITC-RSE) against the PC-3 cell lines, where the cells were exposed to various concentrations in µg/mL of the extract and cisplatin periodically in a time dependent manner. Results showed that the IC50 of GMG-ITC-RSE appeared to be 2.5 µg/mL after 72 h of incubation and was similar to that of cisplatin obtained at the same period shown in Figure 6. However, Figure 7 displays the percentage of the cell's viability after exposure to 0 to 500 µg/mL GMG-ITC-RSE for 24, 48, and 72 h. The data indicated that GMG-ITC-RSE did not have an IC50 value on the 3T3 cell lines due to the inability of the extract to inhibit the growth of 50% of the cells. Moreover, the growth of 37.78% cells was inhibited when exposed to 500 µg/mL GMG-ITC-RSE for 48 h, which was contrary to 72 h (<4%). Figure 5. Comparison of organ weight relative to animal body weight in the test and control groups measured during the necropsy experiment. Results are presented as mean ± SD in percentage. While p < 0.05 is considered significant, similar letters on the means in every cluster indicated no significant (p > 0.05) difference between the two means unless stated otherwise.

Cytotoxicity and Anti-Proliferation Evaluation of GMG-ITC Rich Soluble Extract
The MTT result demonstrated an impressive cytotoxic activity of GMG-ITC rich soluble extract (GMG-ITC-RSE) against the PC-3 cell lines, where the cells were exposed to various concentrations in µg/mL of the extract and cisplatin periodically in a time dependent manner. Results showed that the IC 50 of GMG-ITC-RSE appeared to be 2.5 µg/mL after 72 h of incubation and was similar to that of cisplatin obtained at the same period shown in Figure 6. However, Figure 7 displays the percentage of the cell's viability after exposure to 0 to 500 µg/mL GMG-ITC-RSE for 24, 48, and 72 h. The data indicated that GMG-ITC-RSE did not have an IC 50 value on the 3T3 cell lines due to the inability of the extract to inhibit the growth of 50% of the cells. Moreover, the growth of 37.78% cells was inhibited when exposed to 500 µg/mL GMG-ITC-RSE for 48 h, which was contrary to 72 h (<4%). Results are presented as mean ± SD percentage viability. While p < 0.05 is considered statistically significant, similar letters on the means in every cluster indicated no statistically significant (p < 0.05) difference between the two means, unless stated otherwise.

Morphological Assessment of PC3 Cells by Inverted Light Microscope
The PC-3 cell lines were observed under phase contrast using an inverted light microscope for the assessment of plasma membrane integrity, where morphological changes resulting from the  Results are presented as mean ± SD percentage viability. While p < 0.05 is considered statistically significant, similar letters on the means in every cluster indicated no statistically significant (p < 0.05) difference between the two means, unless stated otherwise.

Morphological Assessment of PC3 Cells by Inverted Light Microscope
The PC-3 cell lines were observed under phase contrast using an inverted light microscope for the assessment of plasma membrane integrity, where morphological changes resulting from the

Morphological Assessment of PC3 Cells by Inverted Light Microscope
The PC-3 cell lines were observed under phase contrast using an inverted light microscope for the assessment of plasma membrane integrity, where morphological changes resulting from the GMG-ITC rich soluble extract (GMG-ITC-RSE) and cisplatin treatment in a time dependent manner were recorded. Figure 8g-

Morphological Assessment of Apoptotic Cells by AO/PI Double Staining
AO/PI double staining of PC-3 cell lines clearly differentiated viable, necrotic, and apoptotic cells in the present study. Untreated (control) cells exhibited round greenish nuclei in Figure 9a, indicating healthy and live cells. Secondary necrosis and apoptosis were observed in both 2.5

Discussion
The growing number of studies on pharmacology and epidemiology has exposed the potential health enhancing properties of plant derived natural compounds [3,8,24,49]. Various phytochemicals, particularly glucosinolates and their hydrolytic products (isothiocyanates), have been promising metabolites demonstrating a drastic decrease in the susceptibility to carcinogenesis [39,40]. Glucosinolates and isothiocyanates including, but not limited to, glucoraphanin, benzyl isothiocyanate, phenethyl isothiocyanate, allyl isothiocyanate, and sulforaphane have been used as strong chemotherapeutic and preventive measures against various types of carcinoma [50][51][52][53]. These sorts of phytochemicals and other potent bioactive compounds are believed to have a modulatory effect on the cancer hallmarks such as angiogenesis, metastasis, and tumor cell proliferation through the induction of a cascade of reactions that trigger intracellular ROS generation leading to the downregulation of various antioxidant genes with consequent cell cycle arrest and eventually death via apoptosis [54,55]. Prostate cancer is a complex disease that affects mostly aged individuals, particularly males, and is associated with an increase in the rate of mortality worldwide [56]. The current treatment of human prostate cancer is shouldered on radiation and chemotherapy after surgery [57,58]. Despite several ways of managing the disease, recurrent tumors have always been a concern to cancer patients, distressing a large percentage of individuals undergoing treatment [58]. Therefore, alternative approaches that employ safer plant-derived natural compounds have become a better option [59]. Thus, using a M. oleifera extract rich in glucomoringin and or its hydrolytic product, which have demonstrated promising biological activities against human prostate carcinoma, appears to be a potential substitute to the existing practice believed to be less effective.
Numerous in vivo and in vitro studies have reported on the various anti-tumor activities of extracts of aerial parts of M. oleifera [43,[60][61][62], which have been proven to be non-toxic as reported by Stohs and Hartman [14]. However, the anti-cancer properties of the glucomoringin rich soluble extract against human prostate carcinoma remained ambiguous. As such, this study reported on the toxicity and antitumor effects of glucomoringin-isothiocyanate rich soluble extracts (GMG-ITC-RSE) by employing inbreed specific pathogen free (SPF) female Sprague-Dawley (SD) rats, murine fibroblast cell lines (3T3), and human prostate adenocarcinoma cell lines (PC-3) as apparently normal and disease models, respectively. After examining the potential toxicological effects of the aqueous GMG-ITC-RSE of M. oleifera seeds on SD rats, where oral doses of up to 2000

Discussion
The growing number of studies on pharmacology and epidemiology has exposed the potential health enhancing properties of plant derived natural compounds [3,8,24,49]. Various phytochemicals, particularly glucosinolates and their hydrolytic products (isothiocyanates), have been promising metabolites demonstrating a drastic decrease in the susceptibility to carcinogenesis [39,40]. Glucosinolates and isothiocyanates including, but not limited to, glucoraphanin, benzyl isothiocyanate, phenethyl isothiocyanate, allyl isothiocyanate, and sulforaphane have been used as strong chemotherapeutic and preventive measures against various types of carcinoma [50][51][52][53]. These sorts of phytochemicals and other potent bioactive compounds are believed to have a modulatory effect on the cancer hallmarks such as angiogenesis, metastasis, and tumor cell proliferation through the induction of a cascade of reactions that trigger intracellular ROS generation leading to the downregulation of various antioxidant genes with consequent cell cycle arrest and eventually death via apoptosis [54,55]. Prostate cancer is a complex disease that affects mostly aged individuals, particularly males, and is associated with an increase in the rate of mortality worldwide [56]. The current treatment of human prostate cancer is shouldered on radiation and chemotherapy after surgery [57,58]. Despite several ways of managing the disease, recurrent tumors have always been a concern to cancer patients, distressing a large percentage of individuals undergoing treatment [58]. Therefore, alternative approaches that employ safer plant-derived natural compounds have become a better option [59]. Thus, using a M. oleifera extract rich in glucomoringin and or its hydrolytic product, which have demonstrated promising biological activities against human prostate carcinoma, appears to be a potential substitute to the existing practice believed to be less effective.
Numerous in vivo and in vitro studies have reported on the various anti-tumor activities of extracts of aerial parts of M. oleifera [43,[60][61][62], which have been proven to be non-toxic as reported by Stohs and Hartman [14]. However, the anti-cancer properties of the glucomoringin rich soluble extract against human prostate carcinoma remained ambiguous. As such, this study reported on the toxicity and antitumor effects of glucomoringin-isothiocyanate rich soluble extracts (GMG-ITC-RSE) by employing inbreed specific pathogen free (SPF) female Sprague-Dawley (SD) rats, murine fibroblast cell lines (3T3), and human prostate adenocarcinoma cell lines (PC-3) as apparently normal and disease models, respectively. After examining the potential toxicological effects of the aqueous GMG-ITC-RSE of M. oleifera seeds on SD rats, where oral doses of up to 2000 mg/kg body weight were administered to the rats in the test group and continuing test group for 14 days. We noticed that there were neither observed overt adverse reactions at this dose nor was any organ enlargement found when the major organs underwent necropsy. Thus, our findings were in line with what was reported by Stohs and Hartman [14], where they considered not only the seeds of M. oleifera, but all aerial parts of the plant as nontoxic and safe enough to move on with clinical studies after the exploration in all respects. The authors gathered that numerous studies on human and animal models have evaluated the general safety of M. oleifera aerial parts, particularly the leaves and seeds, thus demonstrating a high level of safety. Their reports explained how safe the leaves of the plant were when administered in humans as the result revealed no adverse effect on doses of up to 50 g [63]. Similarly, oral administration of up to 8 g/day of the leaf powder for 40 days recorded no toxic effects [64]. Although M. oleifera extracts of any solvents has yet to be tested in humans, in-depth clues on the safety of extracts of different solvents were obtained when the extracts were administered in various animal models. Approximately, 300 mg per kg body weight of M. oleifera leaves and seeds in aqueous extracts, which was equivalent to 3.9 g per 80 kg human BW, was administered in rats [43]. The findings showed that extracts of the M. oleifera aerial parts could be very safe in addition to their potential physiological and pharmacological effects.
We realized that the soluble extract offered an anticancer effect through the induction of morphological alterations and apoptosis in a time dependent manner. After treatment of the PC-3 cells with GMG-ITC-RSE and incubation for 24, 48, and 72 h, we observed noticeable morphological changes at 72 h of incubation. We also observed a deeply stained nuclei and condensed chromatin, which is an indication of the processes that dying cells undergo. The acridine orange (AO) and propidium iodide (PI) double stain confirmed the chromatin condensation and other features peculiar to particular stages of processes for cell death through apoptosis as seen clearly in Figure 8. A similar trend of activity was reported when pancreatic cancer cell lines [65] and human brain glioblastoma multi form cells [66] were incubated with BITC, and glioma cells with PEITC [53], where the PEITC was found to significantly potentiate the induction of cytotoxicity and apoptosis in glioma cells through the agonistic effect of the tumor necrosis factor-related apoptosis-induced ligand (TRAIL).
Although our study did not establish the specific pathway(s) for the signal transduction in the apoptosis process due to some limitations, the accumulated number of findings suggested certain mechanisms through which M. oleifera extracts influence cellular proliferation and the number of genes/signaling proteins involved [67,68]. Meanwhile, according to Jung [43], the soluble extract of M. oleifera remarkably upregulated the expression of cleaved caspase 3 with the consequent downregulation of caspase 3 when treated with lung and other cancer cell lines at different doses. Furthermore, the extract was reported to have triggered apoptosis through the activation of phosphor-extracellular signal related kinase (p-ERK) and phosphor-c-Jun N-terminal kinase (pJNK) in human melanoma cancer cells (A2058) [69]. Similarly, an extract from M. oleifera seeds caused an increase in the phosphorylation level of ERK1/2 and p38 MAPK, which led to apoptosis in RMCCA1 [70]. The authors suggested that the activities of pro-and anti-apoptotic markers could be the benchmark for the apoptotic nature of the extract.
However, studies have shown that the major worrying factor in the drug development process and discovery is the water insolubility of potential anti-cancer agents due to the presence of bulky hydrophobic moieties in their structures [71,72], which reflect difficulties in biotransformation with eventual therapeutic challenges [73]. The more soluble the compounds are, the better their efficacy in cancer treatment and prevention will be. As such, the high polarity of glucosinolate hydrolysis products qualify it to dissolve easily in water, which gives it immense anti-tumor activity [74] in the presence of other related compounds as was mentioned earlier.

Conclusions
We demonstrated the in vitro and in vivo acute toxicity coupled with in vitro anti-tumor activity of a nontoxic glucomoringin-isothiocyanate rich soluble extract from M. oleifera seeds on human prostate adenocarcinoma through proliferative inhibition and apoptotic induction in PC-3 cells. The findings suggested that the soluble extract of M. oleifera seeds possessed a high level of safety and promising physiological and pharmacological activities. The plant could be a potential source of a solution to the challenges facing the current treatment of prostate cancer and perhaps other types of cancer conditions. The highly noticeable antitumor activity of the soluble extract could be attributed to the synergistic effect of GMG-ITC with other potent compounds in the extract that may enhance its activities. However, in-depth investigation at the cellular and molecular level are essential to address the points brought up in the discussion section.