Growth, Yield, and Water Productivity Responses of Pepper to Sub-Irrigated Planter Systems in a Greenhouse

: A sub-irrigated planter (SIP) is a container irrigation technique in which water is supplied to the crop from the bottom, stored in a saturated media-ﬁlled reservoir beneath an unsaturated soil, and then delivered by capillary action to the root zone. The aim of this study was to optimize the water management and to assess the performance of this technique in terms of water use e ﬃ ciency, soil moisture, and solute distribution in comparison with surface irrigation in a Mediterranean greenhouse. The experiment consisted of four SIP treatments, with a constant water level in the bottom reservoir in order to evaluate the e ﬀ ect of two di ﬀ erent irrigation salinities (1.2 and 2.2 dS m − 1 ) and two depths of substrate proﬁles (25 and 15 cm). The results showed that SIP is capable of signiﬁcantly improving both water-use e ﬃ ciency and plant productivity compared with surface irrigation. Also, a 24% average reduction in water consumption was observed while using SIP. Moreover, SIPs with a higher depth were recommended as the optimum treatments within SIPs. The type of irrigation method a ﬀ ected the salinity distribution in the substrate proﬁle; the highest salinity levels were registered at the top layers in SIPs, whereas the maximum salinity levels for the surface treatments were observed at the bottom layers. SIPs provide a practical solution for the irrigation of plants in areas facing water quality and scarcity problems.


Introduction
In terms of addressing the global water shortage in agriculture, which accounts for 70% of global freshwater withdrawals [1], subsurface irrigation currently represents the most efficient irrigation technique in providing water to plants [2,3]. In subsurface irrigation, water is supplied by creating and maintaining a reservoir below the ground surface. Moisture moves upwards through capillary action, in order to meet the water requirements of the crop. As the water is supplied below the surface, water evaporation is negligible in this method, and thus more water is available for the plant's roots [4].

Study Site
The present study was carried out in a 150 m 2 tunnel greenhouse with a ventilation system and relative humidity and temperature control, located at the University of Almeria (36 • 49' N, 2 • 24' W), Almeria, Spain.
Almeria presents a Mediterranean semi-arid climate, with an average annual rainfall of 260 mm and a wide dry period during the summer months. The average monthly temperatures range from 11.8 • C in January to 26 • C in July [21].
Temperature, relative humidity, and solar radiation data were collected using a HOBO U30 weather station (Onset Computer Corporation, Bourne, MA, USA) installed inside the greenhouse for the entire growing season. The data were downloaded and processed using the HOBOware program (Onset Computer Corporation, Bourne, MA, USA).

Treatments Details
The experimental design was a split-split plot and plot, with three factors (2 × 2 × 2, namely: irrigation technique, depth of growing medium, and water irrigation salt level, respectively) and a total of eight treatments, three randomized complete repetitions, and four plants per pot and per block, giving the plus border plants a total of 128 plants. The combinations of tested factors of the experiment are depicted in Figure 1. The irrigation techniques essayed were the sub-irrigated planter (SIP) and surface irrigation (O). The depth of the growing medium essayed were 25 (D 1 ) and 15 cm (D 2 ). The qualities of water essayed were 1.2 (S 1 ) and 2.2 dS m −1 (S 2 ). Statistical analysis was performed using the SPSS version 16.0 statistical software (SPSS Inc, Chicago, IL, USA). A probability of p < 0.05 was considered significant.

Study Site
The present study was carried out in a 150 m 2 tunnel greenhouse with a ventilation system and relative humidity and temperature control, located at the University of Almeria (36°49' N, 2°24' W), Almeria, Spain.
Almeria presents a Mediterranean semi-arid climate, with an average annual rainfall of 260 mm and a wide dry period during the summer months. The average monthly temperatures range from 11.8 °C in January to 26 °C in July [21].
Temperature, relative humidity, and solar radiation data were collected using a HOBO U30 weather station (Onset Computer Corporation, Bourne, MA, USA) installed inside the greenhouse for the entire growing season. The data were downloaded and processed using the HOBOware program (Onset Computer Corporation, Bourne, MA, USA).

Treatments Details
The experimental design was a split-split plot and plot, with three factors (2 × 2 × 2, namely: irrigation technique, depth of growing medium, and water irrigation salt level, respectively) and a total of eight treatments, three randomized complete repetitions, and four plants per pot and per block, giving the plus border plants a total of 128 plants. The combinations of tested factors of the experiment are depicted in Figure 1. The irrigation techniques essayed were the sub-irrigated planter (SIP) and surface irrigation (O). The depth of the growing medium essayed were 25 (D1) and 15 cm (D2). The qualities of water essayed were 1.2 (S1) and 2.2 dS m −1 (S2). Statistical analysis was performed using the SPSS version 16.0 statistical software (SPSS Inc, Chicago, IL, USA). A probability of p < 0.05 was considered significant.

Planters and Plant Details
Pepper (Capsicum annuum) was planted in an experimental greenhouse at the University of Almeria for a growing season lasting from June to September 2019. The seedlings were purchased from a local nursery and were ready to be transplanted into pots. The pots presented holes on the bottom and measured 30 cm in diameter and 30 cm in height (21.2 L). Half of the pots were used for surface irrigation (O) and half of them for sub-irrigated planter (SIP). The surface pots were filled only with a growing medium, which was controlled by the weight. In order to construct the SIP

Planters and Plant Details
Pepper (Capsicum annuum) was planted in an experimental greenhouse at the University of Almeria for a growing season lasting from June to September 2019. The seedlings were purchased from a local nursery and were ready to be transplanted into pots. The pots presented holes on the bottom and measured 30 cm in diameter and 30 cm in height (21.2 L). Half of the pots were used for surface irrigation (O) and half of them for sub-irrigated planter (SIP). The surface pots were filled only with a growing medium, which was controlled by the weight. In order to construct the SIP systems, the pots were divided into two parts, namely: reservoir below and unsaturated growing media above ( Figure 2). A layer of gravel was used at the bottom of each SIP pot as a reservoir (1.5 cm gravel layer for deeper depth and 1 cm layer for the lower growing medium depth), the use of gravel in reservoirs creates a medium that is porous and permeable so that it both holds a substantial volume of water and can be quickly filled. systems, the pots were divided into two parts, namely: reservoir below and unsaturated growing media above (Figure 2). A layer of gravel was used at the bottom of each SIP pot as a reservoir (1.5 cm gravel layer for deeper depth and 1 cm layer for the lower growing medium depth), the use of gravel in reservoirs creates a medium that is porous and permeable so that it both holds a substantial volume of water and can be quickly filled.

Growing Medium Details
The growing media used was a mixture of cocopeat and perlite 3:1 v/v [22,23]. Growers typically utilize this soilless mixture in greenhouses for two main reasons, namely: (1) using lower bulk density materials allows for containers to be easily relocated [24], and (2) ample airspace is provided to ensure adequate gas exchange and to minimize the risk of diseases. The bulk density, particle density, and total porosity values were 0.13, 0.47 g cm −3 , and 72%, respectively, for the soilless growing medium.

Irrigation, Leaching, and Fertilizing Scheduling
After transplanting, all of the plants received surface irrigation for one week to help plant growth. Then, the irrigation scheduling was applied. Under the SIP system, the water level was kept constant by trays placed below each pot and the surface of this tray was completely covered so as to avoid evaporation. The water in the trays moves through the holes at the bottom of the pot to the gravel reservoir and rises by capillary action to the substrate. The level of water was maintained at 1.5 and 1 cm in the gravel layer throughout the entire growing season by adding water to the trays daily and recording the volume applied. This volume was considered the water absorbed by plants per pot, because SIP systems are closed. The water was applied manually under surface irrigation. The daily volume applied was the same volume applied in the SIP plus the volume corresponding to free drainage of the pot.
Considering that the pepper salinity tolerance level is 1 dS m −1 for irrigation water salinity [19][20][21], two qualities of water were obtained by the addition of NaCl to water irrigation (Table 1) to rise the Electrical Conductivity (EC) of 1.2 and 2.2 dS m −1 . Also, a nutrient solution (Table 1) was applied fortnightly at a rate of 70 mL per plant in both irrigation systems, to allow for a more efficient use of both water and fertilizers [22,23].

Growing Medium Details
The growing media used was a mixture of cocopeat and perlite 3:1 v/v [22,23]. Growers typically utilize this soilless mixture in greenhouses for two main reasons, namely: (1) using lower bulk density materials allows for containers to be easily relocated [24], and (2) ample airspace is provided to ensure adequate gas exchange and to minimize the risk of diseases. The bulk density, particle density, and total porosity values were 0.13, 0.47 g cm −3 , and 72%, respectively, for the soilless growing medium.

Irrigation, Leaching, and Fertilizing Scheduling
After transplanting, all of the plants received surface irrigation for one week to help plant growth. Then, the irrigation scheduling was applied. Under the SIP system, the water level was kept constant by trays placed below each pot and the surface of this tray was completely covered so as to avoid evaporation. The water in the trays moves through the holes at the bottom of the pot to the gravel reservoir and rises by capillary action to the substrate. The level of water was maintained at 1.5 and 1 cm in the gravel layer throughout the entire growing season by adding water to the trays daily and recording the volume applied. This volume was considered the water absorbed by plants per pot, because SIP systems are closed. The water was applied manually under surface irrigation. The daily volume applied was the same volume applied in the SIP plus the volume corresponding to free drainage of the pot. Considering that the pepper salinity tolerance level is 1 dS m −1 for irrigation water salinity [19][20][21], two qualities of water were obtained by the addition of NaCl to water irrigation (Table 1) to rise the Electrical Conductivity (EC) of 1.2 and 2.2 dS m −1 . Also, a nutrient solution (Table 1) was applied fortnightly at a rate of 70 mL per plant in both irrigation systems, to allow for a more efficient use of both water and fertilizers [22,23].

Crop and Potential Evapotranspiration
Reference evapotranspiration was calculated using the Penman-Monteith equation as a function of the registered climatic variables. Crop evapotranspiration was calculated by applying a daily water balance equation to each pot (See Equation (1)).
where: ET c = crop evapotranspiration, V I = is the daily irrigation water applied, V D = is the irrigation water drained and ∆V S is the variation of the water content in the substrate. For SIP pots V D was equal to zero and ∆V S was neglected in a daily basis.

Water Use Efficiency, Moisture, and Solute Distributions
Water use efficiency, defined as the irrigation water volume required to produce a given total mass of yield, was estimated and compared for all of the treatments at the end of the growing season. All of the planters were divided into 5 cm layers, and the volumetric moisture contents and salinity levels of these 5 cm growing medium layers were measured and monitored throughout the season. The volumetric moisture content was measured by the gravimetric method; the salinity levels were measured by collecting the growing medium water extract and then measuring the salinities using an EC-meter.

Greenhouse Meteorological Data
The meteorological data inside the greenhouse effect plant growth. Thus, it is essential to gather such data in order to measure the potential evapotranspiration and crop coefficient. Figure 3 illustrates the meteorological data, including temperature, relative humidity, and solar radiation over the course of the research period. The temperature and relative humidity ranged from 30.9 to 32.2 • C and 88% to 98%, respectively. The peak solar radiation was reached in August.

Plant Productivity and Water Use Efficiency (WUE)
The growth of the pepper plants used in this study was assessed in terms of dry and fresh yield weights, height of plants' canopy, and number of leaves ( Table 2). All of these growth parameters were noticeably different between the sub-irrigated planter and surface treatments. The values of these parameters were considerably lower in the surface irrigation than in the SIP. Within the SIP treatment; the most significant factor affecting the growth of the crop was the depth of the substrate profile, whereas the irrigation water salinity level did not affect the growth parameters (Tukey's test, p < 0.05). The results are shown in Table 2.
The meteorological data inside the greenhouse effect plant growth. Thus, it is essential to gather such data in order to measure the potential evapotranspiration and crop coefficient. Figure 3 illustrates the meteorological data, including temperature, relative humidity, and solar radiation over the course of the research period. The temperature and relative humidity ranged from 30.9 to 32.2 °C and 88% to 98%, respectively. The peak solar radiation was reached in August.    The total irrigation during the growing season was higher in the treatments with a higher substrate depth than in those with a lower depth. While such an observation is reasonably to be expected, the most notable finding in the trial was the substantial reduction in the total irrigation figures when using SIPs; 19%, 28%, 21%, and 27% total irrigation declines were observed in SIPs compared with surface treatments, respectively (Table 2).

Number of Leaves
SIPs had the highest water use efficiency figures in all of the treatments compared with the surface treatments. The maximum WUE value was seen in SIP-S1-D2, reaching 4.99 gr L −1 , which was 1.83 times higher than O-S1-D2. This SIP treatment also had the maximum WUE among all SIPs, although the figures showed no significant difference in p < 0.05 within the SIP treatments. Table 3 shows the effect of irrigation treatment on plant canopy (stem and leaves) and root systems. According to this table, the SIP significantly increased the root and canopy weights compared with surface irrigation. To compare the SIP treatments, Table 3 showed that water salinity levels did not affect the canopy and root weights between the SIP-S1 and SIP-S2 treatments; although the depth caused a remarkable difference and the SIP-D1 treatments experienced considerably greater fresh root weights than SIP-D2s. The canopies also had noticeably greater weight values in the SIP than the surface treatments; although they did not differ significantly among SIPs. Fresh canopy/root ratios did not substantially differ among all of the treatments; except for figures in O-S1-D2 and O-S2-D2 treatments, which were far higher than the SIPs. 3.3. The Culture Evapotranspiration (ETc) and Reference Evapotranspiration (ET 0 ) Data Table 4 presents a summary of the actual evapotranspiration measurements taken at the initial, developing, middle, and late stages of the study, as well as the estimated (ET 0 ) and crop coefficient (Kc) values for pepper over the course of the growth period. ETc reached its highest level in the SIP-S1-D1 treatment during the middle stage. At the initial stage, the ETc figures (and Kc values logically), did not differ significantly between the SIP and surface treatments. At the developing stage, the results were also the same between the SIP and surface treatments; whereas among the SIPs, the figures were higher in SIPs-D1 compared with SIPs-D2. At the middle stage, only the ETc values for SIP-S2-D2 and O-S2-D2 differed considerably. Finally, the ETc figures were far higher in SIP-S1-D1 than O-S1-D1, and in O-S1-D2 than SIP-S1-D2 at the late growth stage.

Moisture and Solute Distributions
In order to study the growing media profiles, Figure 4 shows the volumetric moisture content distributions during the entire growing season. In the case of the SIP treatments, the lowest soil moisture occurred in the surface layers, and the moisture increased downwards. A maximum soil moisture was reached at the interface between the substrate and the saturated reservoir at the bottom of the container. The soil moisture profiles were different in the SIP and O treatments. The soil moisture was always lower in the surface layer in all of the SIP treatments than in their corresponding surface treatments, whereas at deeper layers, the soil moisture content was lower in the O treatments (Figure 4a,b). The irrigation water salinity levels influenced the water flow and the soil moisture content in the soil profile. The treatments irrigated with more saline water had a higher soil moisture. For example, the soil moisture in treatment SIP-S2 was higher than treatment SIP-S1 throughout the soil profile. The water flow was higher for the lower substrate depth (D2), so these treatments displayed a higher soil moisture than the D1 treatments (Figure 4c). Figure 5 depicts the salinity distributions in the substrate for different treatments. The salinity distribution was completely different in the SIP and O treatments (Figure 5a,b). The highest salinity levels were registered at the top layers in the SIPs, whereas the maximum salinity levels for the surface treatments were observed at the bottom layers. This behavior was repeated for both substrate depths, D1 and D2. As was expected, both for the SIP and surface irrigation, the S2 treatments showed higher salinity levels in the entire substrate profile than the S1 treatments. The depth of the substrate profile also affected the salinity distribution. As can be observed (Figure 5c), the deeper the soil profile (treatment D1), the higher the saline levels were in the substrate.
Sustainability 2019, 11, x FOR PEER REVIEW 8 of 14 displayed a higher soil moisture than the D1 treatments (Figure 4c). Figure 5 depicts the salinity distributions in the substrate for different treatments. The salinity distribution was completely different in the SIP and O treatments (Figure 5a,b). The highest salinity levels were registered at the top layers in the SIPs, whereas the maximum salinity levels for the surface treatments were observed at the bottom layers. This behavior was repeated for both substrate depths, D1 and D2. As was expected, both for the SIP and surface irrigation, the S2 treatments showed higher salinity levels in the entire substrate profile than the S1 treatments. The depth of the substrate profile also affected the salinity distribution. As can be observed (Figure 5c), the deeper the soil profile (treatment D1), the higher the saline levels were in the substrate.  SIP systems must be managed precisely in order to avoid saturating the soil during the growing season. The soil surface should be monitored to prevent high salinity levels at the top layers, and, if necessary, leaching should be applied. With due attention to the abovementioned points, Figures 6  and 7 provide the average volumetric moisture contents and soil salinity levels over the course of the growing period. In all of the SIP-D1 treatments, deeper layers (fourth and fifth layers) were consistently near saturation and considerably wetter than the same layers in the O-D1 treatments. However, the SIP-D1 first and second layers continued to be drier than those of the surface irrigation (Figure 6a,b). For the SIP-D2 treatments, the third and first layers had higher and lower moisture displayed a higher soil moisture than the D1 treatments (Figure 4c). Figure 5 depicts the salinity distributions in the substrate for different treatments. The salinity distribution was completely different in the SIP and O treatments (Figure 5a,b). The highest salinity levels were registered at the top layers in the SIPs, whereas the maximum salinity levels for the surface treatments were observed at the bottom layers. This behavior was repeated for both substrate depths, D1 and D2. As was expected, both for the SIP and surface irrigation, the S2 treatments showed higher salinity levels in the entire substrate profile than the S1 treatments. The depth of the substrate profile also affected the salinity distribution. As can be observed (Figure 5c), the deeper the soil profile (treatment D1), the higher the saline levels were in the substrate.  SIP systems must be managed precisely in order to avoid saturating the soil during the growing season. The soil surface should be monitored to prevent high salinity levels at the top layers, and, if necessary, leaching should be applied. With due attention to the abovementioned points, Figures 6  and 7 provide the average volumetric moisture contents and soil salinity levels over the course of the growing period. In all of the SIP-D1 treatments, deeper layers (fourth and fifth layers) were consistently near saturation and considerably wetter than the same layers in the O-D1 treatments. However, the SIP-D1 first and second layers continued to be drier than those of the surface irrigation (Figure 6a,b). For the SIP-D2 treatments, the third and first layers had higher and lower moisture SIP systems must be managed precisely in order to avoid saturating the soil during the growing season. The soil surface should be monitored to prevent high salinity levels at the top layers, and, if necessary, leaching should be applied. With due attention to the abovementioned points, Figures 6 and 7 provide the average volumetric moisture contents and soil salinity levels over the course of the growing period. In all of the SIP-D1 treatments, deeper layers (fourth and fifth layers) were consistently near saturation and considerably wetter than the same layers in the O-D1 treatments. However, the SIP-D1 first and second layers continued to be drier than those of the surface irrigation (Figure 6a,b). For the SIP-D2 treatments, the third and first layers had higher and lower moisture contents, respectively, than those of the surface irrigation throughout the season (Figure 6c,d). Regarding the salinity levels, in the wetter layers in Figure 6, the salinity levels tended to be lower, whereas in the drier layers, they were noticeably higher (Figure 7), because the wetter the soil, the more diluted the salts are. As can be seen, the salinity levels in the root area exceeded the plant's tolerance limit in the SIP-D1 treatments (Figure 7a,b). By contrast, the SIP-D2 treatments kept the salinity level near the tolerance limit throughout the entire study period (Figure 7c,d).
Sustainability 2019, 11, x FOR PEER REVIEW 9 of 14 contents, respectively, than those of the surface irrigation throughout the season (Figure 6c,d).
Regarding the salinity levels, in the wetter layers in Figure 6, the salinity levels tended to be lower, whereas in the drier layers, they were noticeably higher (Figure 7), because the wetter the soil, the more diluted the salts are. As can be seen, the salinity levels in the root area exceeded the plant's tolerance limit in the SIP-D1 treatments (Figure 7a,b). By contrast, the SIP-D2 treatments kept the salinity level near the tolerance limit throughout the entire study period (Figure 7c,d).

Discussion
Based on the results obtained, there was a significant difference between the SIP and surface treatments, as well as among the SIP treatments subjected to different salinities and depths. Particular emphasis should be placed on the WUE figures (Table 2), which were consistently higher in the SIPs than in their corresponding surface treatments. Lee [25] also proved that the capillary wick container systems improve water and nutrient solution efficiency in relation to conventional surface irrigation systems.
In this study, the maximum WUE was reported for treatment SIP-S1-D2 with 4.99 g L −1 , while O-S2-D1 experienced the minimum WUE (2.31 g L −1 ). Nalliah [10] reported 9.33 g L −1 as the maximum WUE for the capillary negative pressure container systems, compared with 6.27 g L −1 for the hand watered pots in a study to evaluate the negative pressure capillary irrigation effect on hot pepper's quality in a greenhouse.
WUE was notably improved in the SIPs, and the reason was not only the higher plant productivity (yield), but also the lower irrigation consumption in SIP treatments compared with the surface. To determine what the main factors are for producing this result, both the plant

Discussion
Based on the results obtained, there was a significant difference between the SIP and surface treatments, as well as among the SIP treatments subjected to different salinities and depths. Particular emphasis should be placed on the WUE figures (Table 2), which were consistently higher in the SIPs than in their corresponding surface treatments. Lee [25] also proved that the capillary wick container systems improve water and nutrient solution efficiency in relation to conventional surface irrigation systems.
In this study, the maximum WUE was reported for treatment SIP-S1-D2 with 4.99 g L −1 , while O-S2-D1 experienced the minimum WUE (2.31 g L −1 ). Nalliah [10] reported 9.33 g L −1 as the maximum WUE for the capillary negative pressure container systems, compared with 6.27 g L −1 for the hand watered pots in a study to evaluate the negative pressure capillary irrigation effect on hot pepper's quality in a greenhouse.
WUE was notably improved in the SIPs, and the reason was not only the higher plant productivity (yield), but also the lower irrigation consumption in SIP treatments compared with the surface. To determine what the main factors are for producing this result, both the plant productivities and total irrigation values need to be evaluated first ( Table 2). As can be observed in Table 2, all of the growth parameters were significantly greater in every SIP treatment compared with its equivalent surface treatment, suggesting that the SIP treatments provided a better aeration of the root system and a higher water supply to meet the needs of the plants. This adequate supply of air and water stems from a correct combination of growing medium and irrigation techniques. Water in the SIP systems is supplied constantly through capillary action, and the substrate contains cocopeat, which has a very high water holding capacity to retain this water. Also, the incorporation of perlite increases aeration and avoids saturation in SIP. Therefore, this mixture constitutes an optimum growing media to keep water and air available over the course of the growing season [26]. In addition, SIP systems use water very efficiently, as direct evaporation from the soil surface is considerably reduced and water is not lost in drainage. In surface irrigation, on the contrary, water can be lost by both evaporation and drainage. These factors simultaneously led to a better plant yield and less water consumption in the SIP than the surface.
The results ( Table 2) proved that increasing the irrigation salinity levels in SIPs (S1 vs. S2) as a container system did not significantly reduce either the growth parameters or the WUE, which coincides with Caparros et al. [27], who evaluated the water and nutrient uptake efficiency in the containerized production of lavender irrigated with saline water. This may be due to the fact that the growing medium is kept wetted enough to dilute the salts, and thus overcome the negative effects of the salinity.
Regarding the effect of the substrate depth in SIP irrigation ( Table 2), all of the growth parameters were lower in D2 than in D1. In contrast, the WUE was not affected, as the total irrigation volume was accordingly lower. It is assumed that the lower depth in the SIP systems could not maintain the sufficient aeration needed for the roots, resulting in less plant productivity.
The results showed that SIP significantly improved the plant root system in terms of its weight in comparison to surface irrigation (Table 3). These findings resemble those obtained by Al-Harbi et al. [28] in their study on the effect of sub surface drip irrigation on Okra growth. This could be attributed to a better balance of water and air in the root zone using SIP, which enhanced the growth of the root system compared with surface irrigation, which tended to raise the moisture in the top layer. Another result worth mentioning is the effect of the depth of the substrate profile on root growth in the SIPs. Deeper SIP profiles yielded the most extensive and dense rooting system. This effect may be due to the larger available space in the deeper growing media, which stimulates the growth and development of the root system in the presence of optimum air and moisture distributions. Canopy weights were also significantly higher in the SIPs than in the surface irrigation (Table 3), as, in SIP, the canopy is not irrigated, which protects this part of the plant from diseases and helps it to be healthier and more well developed. Dastorani et al. [29] reported similar results on the effect of sub surface irrigation on canopy development. The fresh canopy/root ratio was higher in the O-D2 treatments, which shows that as surface irrigation uses more water than SIP, a shallow and poor root system is developed in non-limiting water conditions. ETc was significantly greater in the SIP-D1 treatments compared with SIP-D2 at the developing, middle, and late growth stages (Table 4). According to the FAO56 guidelines, extra water in the root zone produces poor root respiration and less water and nutrition absorption, which may be the main reason for the lower ETc in the SIP-D2 treatments. Applying 15 cm of water in the SIP-D 2 treatments is not enough to meet the ETc requirements for the crop, which may be the main reason for the lower ETc value in the SIP D 2 treatments. Taking into account the greater plant productivity and growth parameters in SIP-D1 than in SIP-D2, the importance of depth in a SIP system is revealed. It suggests a depth selection guideline for SIP users; in areas facing water scarcity, where water saving is a priority and plant productivity is a secondary objective, a lower SIP depth is recommended. On the contrary, it would be better to apply deeper SIPs for expensive ornamental or horticulture plants, whose marketability is of the utmost importance. When comparing surface and SIPs (Table 4), the ETc values were not considerably different at the beginning of the growing season, while at the late stage, they were significantly higher in the SIP-S1-D1 than in the O-S1-D1, and lower in the SIP-S1-D2 than in the O-S1-D2. These differences are from the abovementioned reasons; indeed, better water delivery and aeration takes place in the deepest root zone in SIP-S1-D1, and SIP-S1-D2 has poorer aeration. This behavior suggests the compatibility of using shallower depths in surface irrigation than in SIP.
The moisture content was greater in the surface treatment top layers than the SIPs (Figure 4). In SIPs, the wetter layers were located at the bottom of pots, which is logical, because of the reservoir presence at the bottom of SIP pots. All of the layers of SIP-D2 had a higher moisture content than SIP-D1 (Figure 4c), although they did not reach the saturation point. These findings highlight the need to do regular moisture monitoring of SIPs with lower depths. Another remarkable point about the same graph is the higher moisture contents in the SIP-S2 treatments compared with those of SIP-S1. Despite differing slightly, which occurred in both the D1 and D2 treatments, it can be concluded that applying saline water increases the moisture content, which is also reported in previous studies about moisture and salinity relationships [30]. FAO 56 explains that the reason for this may lie in the accumulation of salts, such as sodium, which causes the destruction of growing media's structure and plants' poor water absorption.
The highest salinity levels were observed in the top SIP layers, as well as in the deepest surface layers ( Figure 5). In the surface systems, salts flowed down to deeper layers, while in SIP, salts accumulated in the drier surface layers.
It is observed that the moisture content in all of the substrate layers for the SIP treatments remained almost constant all over the growing season, with moderate fluctuations ( Figure 6). In SIP, water is utilized through evaporation (which is limited in SIP) and transpiration, and the creation of a matric suction in the top layers maintains continuous capillary flow from the reservoir. SIP techniques may be easy to install and maintain, although they need precise management, depending on the meteorological data and plant absorption, so as to keep a constant flow distribution. It is necessary to maintain substrate moisture within limits, without reaching saturation conditions in the bottom layers, and the moisture must also not be lower than the allowable water depletion in the top layers throughout the growing season. It is recommended to monitor the moisture content daily when using constant reservoirs, or replace this design with SIPs without a constant reservoir (to fill reservoir at intervals), which are suitable for less water consuming plants, fine texture soils, or where there is limited access to water on daily basis.
As applying saline water using surface irrigation causes leaf burn and injures the plant canopy, the literature has advised using sub-irrigation methods to apply saline water [31,32]. Nevertheless, it should be taken into account that sub-irrigation systems may increase the salinity in the top layers, and monitoring the salinity levels while using them is unavoidable. The salinity levels in the first and second layers in the SIP-D1 treatments were always higher than in the surface irrigation throughout the entire season (Figure 7a,b). Their values exceeded the pepper salinity tolerance limit, so a mild leaching was applied at the middle of the growing season. In contrast, the third, fourth, and fifth SIP-D1 layers maintained salinity within the allowable range. Comparing SIP-D1 and D2 treatments (Figure 7a vs. Figure 7c; Figure 7b vs. Figure 7d), it should be noted that SIPs with lower depths of substrate are more appropriate for more saline irrigation waters, because the soil profile was wetter in this case and the salinity levels were maintained lower.

Conclusions and Recommendations
Fresh water scarcity will be one of the most challenging issues in agriculture, which drives irrigation specialists to promote effective and practical irrigation methods to be used in both urban and industrial greenhouses. From this point of view, the present study evaluated the performance of sub-irrigated planters-a new promising irrigation system in terms of water conservation and plant productivity. SIP irrigation was compared to surface irrigation for four treatments, comprising two different substrate depths and two different water salinities.
The results proved that SIP outperformed surface irrigation in terms of both growth parameters and WUE. All of the SIP treatments improved WUE compared with those of the surface. In this research, the SIP-D2 treatments reached the maximum WUE values out of the SIPs, although they recorded lower yields than the SIP-D1 treatments. Therefore, it is concluded that SIP-D1 treatments are the optimum treatments for SIPs in term of improving WUE and yield simultaneously. SIP methods can be utilized when there is no access to fresh water, which was achieved in this study, as water salinity levels do not affect their performance. SIP methods require neither complicated design nor professional installation; moreover, they outperform in-ground sub-irrigation systems and standard raised beds as they are portable, which would benefit tenants.
This study suggests that SIP is a useful technique for growing pepper plants in greenhouses; however, in the continuation of this trial and in future studies, more depths and crops should be tested to find the best depth guidelines for different ranges of crops. It is also recommended that the SIP studies be conducted for two or more growing seasons so as to study their performance and effectiveness over time.